Lysobacter spongiae sp. nov., isolated from spongin

• Published in: The journal of microbiology Vol. 56; no. 2; pp. 97 - 103
• Main Authors: Choi, Heejae, Im, Wan-Taek, Park, Jin-Sook
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.02.2018; Springer Nature B.V; 한국미생물학회
• Subjects: Agar; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Cardiolipin; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Life Sciences; Lipids; Lysobacter - classification; Lysobacter - genetics; Lysobacter - isolation & purification; Lysobacter - physiology; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phospholipids - analysis; Phylogeny; Quinones; Quinones - analysis; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sequencing; Similarity; Soil Microbiology; Species Specificity; 생물학; 16S rRNA gene; polyphasic taxonomy; Lysobacter spongiae
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-018-7462-3

A Gram-negative, motile, aerobic and rod-shaped bacterial strain designated 119BY6-57 T was isolated from spongin. The taxonomic position of the novel isolate was confirmed using the polyphasic approach. Strain 119BY6-57 T grew well at 25–30°C on marine agar. On the basis of 16S rRNA gene sequence similarity, strain 119BY6-57 T belongs to the family Xanthomonadaceae and is related to Lysobacter aestuarii S2-C T (99.8% sequence similarity), L. maris KMU-14 T (97.5%), and L. daejeonensis GH1-9 T (97.3%). Lower sequence similarities (97.0%) were found with all of the other recognized members of the genus Lysobacter . The G + C content of the genomic DNA was 69.9 mol%. The major respiratory quinone was Q-8 and the major fatty acids were C 16:0 iso, C 15:0 iso, summed feature 9 (comprising C 17:1 iso ω 9 c and/or C 16:0 10-methyl), summed feature 3 (comprising C 16:1 ω 7 c and/or C 16:1 ω 6 c ), and C 11:0 iso 3-OH. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified phospholipids, and an unidentified polar lipid. DNADNA relatedness values between strain 119BY6-57 T and its closest phylogenetically neighbors were below 48.0 ± 2.1%. Based on genotypic and phenotypic characteristics, it is concluded that strain 119BY6-57 T is a new member within the genus Lysobacter , for which the name Lysobacter spongiae sp. nov. is proposed. The type strain is 119BY6-57 T (= KACC 19276 T = LMG 30077 T ).