Polymorphisms of microsomal triglyceride transfer protein in different hepatitis B virus-infected patients

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 35; pp. 5454 - 5460
• Main Authors: Yang, Zhi-Tao, Zhang, Xin-Xin, Kong, Xiao-Fei, Zhang, Dong-Hua, Zhang, Shen-Ying, Jiang, Jie-Hong, Gong, Qi-Ming, Jin, Gen-Di, Lu, Zhi-Meng
• Format: Journal Article
• Language: English
• Published: United States The WJG Press and Baishideng 21.09.2008
• Subjects: Adolescent; Adult; Aged; Alleles; Asian Continental Ancestry Group - genetics; Base Sequence; Carrier Proteins - genetics; Child; China; DNA Primers - genetics; Female; Gene Frequency; Hepatitis B - genetics; Hepatitis B, Chronic - genetics; Humans; Linkage Disequilibrium; Male; Middle Aged; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Rapid Communication; Young Adult; 乙型肝炎病毒; 甘油三酸酯转移蛋白; 神经元体细胞; 遗传多样性; China
• ISSN: 1007-9327; 2219-2840; 2219-2840
• DOI: 10.3748/wjg.14.5454

AIM： To identify the two polymorphisms of microsomal triglyceride transfer protein （MTP） gene in the Chinese population and to explore their correlation with both hepatitis B virus （HBV） self-limited infection and persistent infection. METHODS： A total of 316 subjects with self-limited HBV infection and 316 patients with persistent HBV infection （195 subjects without familial history）, matched with age and sex, from the Chinese Han population were enrolled in this study. Polymorphisms of MTP at the promoter region -493 and at H297Q were determined by the allele specific polymerase chain reaction （PCR）. RESULTS： The ratio of males to females was 2.13：1 for each group and the average age in the self-limited and chronic infection groups was 38.36 and 38.28 years, respectively. None of the allelic distributions deviated significantly from that predicted by the Hardy-Weinberg equilibrium. There was a linkage disequilibrium between H297Q and -493G/T （D＇ = 0.77）. As the χ^2 test was used, the genotype distribution of MTP-493G/T demonstrated a significant difference between the self-limited infection group and the entire chronic group or the chronic patients with no family history （χ^2 = 8.543, P = 0.015 and χ^2 = 7.199, P = 0.019）. The allele distribution at the MTP-493 position also demonstrated a significant difference between the study groups without family history （χ^2 = 6.212, P = 0.013）. The T allele emerged as a possible protective factor which may influence the outcomes of HBV infection （OR： 0.59; 95% CI： 0.389-0.897）. CONCLUSION： The polymorphism of the MTP gene, T allele at -493, may be involved in determining the HBV infection outcomes, of which the mechanism needs to be further investigated.