Microsatellite marker development in rose and its application in tetraploid mapping

• Published in: Journal of the American Society for Horticultural Science Vol. 131; no. 3; pp. 380 - 387
• Main Authors: Zhang, L.H, Byrne, D.H, Ballard, R.E, Rajapakse, S
• Format: Journal Article
• Language: English
• Published: 01.05.2006
• Subjects: chromosome mapping; genetic markers; genetic polymorphism; hybrids; linkage (genetics); linkage groups; loci; microsatellite repeats; molecular sequence data; polymerase chain reaction; Rosa; Rosa wichuriana; tetraploidy
• ISSN: 0003-1062; 2327-9788
• DOI: 10.21273/jashs.131.3.380

Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crepin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into existing maps of the parents 86-7 and 82-1134, which were constructed primarily with AFLP markers. The current map of the male parent, amphidiploid 86-7, consists of 286 markers assigned to 14 linkage groups and covering 770 CM. The map of the female tetraploid parent, 82-1134, consists of 256 markers assigned to 20 linkage groups and covering 920 CM. Nineteen rose SSR loci were mapped on the 86-7 map and 11 on the 82-1134 map. Several homeologous linkage groups within maps were identified based on SSR markers. In addition, some of the SSR markers provided anchoring points between the two parental maps. SSR markers were also useful for joining small linkage groups. Based on shared SSR markers, consensus orders for four rose linkage groups between parental maps were generated. Microsatellite markers developed in this study will provide valuable tools for many aspects of rose research including future consolidation of diploid and tetraploid rose genetic linkage maps, genetic, phylogenetic and population analyses, cultivar identification, and marker-assisted selection.