Frequencies at three polymorphic sites of interleukin-10 gene promoter in Brazilian renal recipients

• Published in: Transplantation proceedings Vol. 35; no. 8; pp. 2908 - 2910
• Main Authors: Plothow, A, Benvenutti, R, Contieri, F.L.C, Bicalho, M.G
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2003; Elsevier Science
• Subjects: Adolescent; Adult; Base Sequence; Biological and medical sciences; Brazil; Child; European Continental Ancestry Group; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genotype; Humans; Interleukin-10 - genetics; Kidney Transplantation - immunology; Medical sciences; Middle Aged; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; Tissue, organ and graft immunology; South America; Brazil; America; Immune response; Transcription promoter; Cytokine; Transplantation; Homotransplantation; Kidney; Treatment; Urinary system; Gene; Surgery; Interleukin 10; Graft; Frequency; Genetics; Polymorphism
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2003.10.013

We genotyped by PCR-RFLP 141 renal patients (77 men and 64 women with ages between 12 and 58, predominantly Caucasians) for allelic variants of three polymorphic sites of the interleukin-10 gene promoter, previously showed to be associated with different production of IL-10 cytokine: −1082 (G/A), −819 (C/T), and −592 (C/A). These polymorphisms may confer flexibility in the common immune responses and influence the outcome of allo-responses after transplantation. Our aim was to determine the frequencies of three functional polymorphic sites −1082, −819, and −592 of the interleukin-10 gene promoter in renal recipients of Curitiba, Paraná. Paraná State is located in southern Brazil, and its capital is Curitiba with approximately 1.5 million inhabitants. Genotypes were classified as follows: “low” IL-10 producer genotypes (ATA/ATA, ACC/ATA, ACC/ACC), “intermediate” genotypes (GCC/ACC, GCC/ATA), and “high” IL-10 producer genotype (GCC/GCC). In our population we observed linkage disequilibrium between alleles −819C and −592C and this haplotypic combination was more frequent (65%) than −819T and −592A. We found significantly reduced frequency of the genotype and haplotype responsible for high production of interleukin-10, maybe because we have a selected group (only renal patients) or maybe because Brazilian people are very heterogeneous (miscegenational), which may differ from other groups.