Is photobleaching necessary for Raman imaging of bone tissue using a green laser?

• Published in: Biochimica et biophysica acta Vol. 1758; no. 7; pp. 868 - 873
• Main Authors: Golcuk, Kurtulus, Mandair, Gurjit S., Callender, Andrew F., Sahar, Nadder, Kohn, David H., Morris, Michael D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2006
• Subjects: Animals; Bone; Bone and Bones - chemistry; Cattle; Diagnostic Imaging - methods; EMCCD; Fluorescence; Fluorescence Recovery After Photobleaching; Lasers; Minerals - analysis; Photobleaching; Raman imaging; Raman microspectroscopy; Spectrum Analysis, Raman - methods; Raman microspectroscopy; Bone; EMCCD; Raman imaging; Photobleaching

Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of...