Is photobleaching necessary for Raman imaging of bone tissue using a green laser?

Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of...

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Published inBiochimica et biophysica acta Vol. 1758; no. 7; pp. 868 - 873
Main Authors Golcuk, Kurtulus, Mandair, Gurjit S., Callender, Andrew F., Sahar, Nadder, Kohn, David H., Morris, Michael D.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.07.2006
Subjects
Animals
Bone
Bone and Bones - chemistry
Cattle
Diagnostic Imaging - methods
EMCCD
Fluorescence
Fluorescence Recovery After Photobleaching
Lasers
Minerals - analysis
Photobleaching
Raman imaging
Raman microspectroscopy
Spectrum Analysis, Raman - methods
Raman microspectroscopy
Bone
EMCCD
Raman imaging
Photobleaching
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Summary:Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1–5 s range and to obtain Raman images less than in 10 min.
Bibliography:ObjectType-Article-1
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ISSN:0005-2736
0006-3002
1879-2642
DOI:10.1016/j.bbamem.2006.02.022