Is photobleaching necessary for Raman imaging of bone tissue using a green laser?

Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of...

Full description

Saved in:
Bibliographic Details
Published inBiochimica et biophysica acta Vol. 1758; no. 7; pp. 868 - 873
Main Authors Golcuk, Kurtulus, Mandair, Gurjit S., Callender, Andrew F., Sahar, Nadder, Kohn, David H., Morris, Michael D.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.07.2006
Subjects
Animals
Bone
Bone and Bones - chemistry
Cattle
Diagnostic Imaging - methods
EMCCD
Fluorescence
Fluorescence Recovery After Photobleaching
Lasers
Minerals - analysis
Photobleaching
Raman imaging
Raman microspectroscopy
Spectrum Analysis, Raman - methods
Raman microspectroscopy
Bone
EMCCD
Raman imaging
Photobleaching
Online AccessGet full text

Cover

Abstract Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1–5 s range and to obtain Raman images less than in 10 min.
AbstractList Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1–5 s range and to obtain Raman images less than in 10 min.
Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1-5 s range and to obtain Raman images less than in 10 min.
Author Golcuk, Kurtulus
Callender, Andrew F.
Morris, Michael D.
Sahar, Nadder
Kohn, David H.
Mandair, Gurjit S.
Author_xml – sequence: 1
  givenname: Kurtulus
  surname: Golcuk
  fullname: Golcuk, Kurtulus
  organization: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
– sequence: 2
  givenname: Gurjit S.
  surname: Mandair
  fullname: Mandair, Gurjit S.
  organization: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
– sequence: 3
  givenname: Andrew F.
  surname: Callender
  fullname: Callender, Andrew F.
  organization: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
– sequence: 4
  givenname: Nadder
  surname: Sahar
  fullname: Sahar, Nadder
  organization: Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA
– sequence: 5
  givenname: David H.
  surname: Kohn
  fullname: Kohn, David H.
  organization: Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA
– sequence: 6
  givenname: Michael D.
  surname: Morris
  fullname: Morris, Michael D.
  email: mdmorris@umich.edu
  organization: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/16584709$$D View this record in MEDLINE/PubMed
BookMark eNp9UE1Lw0AQXaRiW_UfiOzJW-pksx_JRZHiR0EQRc_LZjOpKUm27iaC_94tLXgTBgZm3nsz783JpHc9EnKRwiKFVF5vFmVpOuwWDEAugMViR2SW5qpImORsQmYAIBKmMjkl8xA2EGmciRMyTaXIuYJiRl5XgW4_3eDKFo39bPo17dFiCMb_0Np5-mY609OmM-vdztW0jG_QoQlhRDqG3dDQtUfsaWsC-tszclybNuD5oZ-Sj4f79-VT8vzyuFrePSeWgxwSJbPMFpXKS5sLBQxyUxhhUlUjlGiZZJk0rBC8BFZbJnJbpZkUheG5NILz7JRc7XW33n2NGAbdNcFi25oe3Ri0zJVUkReBfA-03oXgsdZbH_34H52C3kWpN3ofpd5FqYHFYpF2edAfyw6rP9Ihuwi42QMwuvxu0OtgG-wtVo1HO-jKNf9f-AWrQ4dr
CitedBy_id crossref_primary_10_1021_acs_biochem_7b00262
crossref_primary_10_1016_j_forsciint_2017_10_034
crossref_primary_10_1080_05704928_2017_1400442
crossref_primary_10_1002_jrs_2418
crossref_primary_10_1016_j_rio_2023_100561
crossref_primary_10_1038_s41598_024_61652_9
crossref_primary_10_1016_j_bone_2020_115490
crossref_primary_10_1177_0003702819881762
crossref_primary_10_3390_bioengineering5040079
crossref_primary_10_22463_2011642X_3308
crossref_primary_10_1016_j_jhazmat_2023_132462
crossref_primary_10_1016_j_jphotobiol_2016_10_035
crossref_primary_10_1039_b822354h
crossref_primary_10_3389_fmars_2018_00170
crossref_primary_10_1002_jrs_5010
crossref_primary_10_1021_ac103259v
crossref_primary_10_1111_pala_12270
crossref_primary_10_1364_OE_23_023935
crossref_primary_10_1117_1_JBO_17_7_077006
crossref_primary_10_3390_ijms231810689
crossref_primary_10_1364_OE_21_031632
crossref_primary_10_1016_j_bios_2014_10_082
crossref_primary_10_1016_j_forsciint_2021_111087
crossref_primary_10_1088_1361_6501_abb044
crossref_primary_10_1016_j_foodcont_2018_08_017
crossref_primary_10_1039_D1AN01560E
crossref_primary_10_1002_cem_1027
crossref_primary_10_1016_j_vibspec_2014_06_007
crossref_primary_10_1109_TIM_2021_3054679
crossref_primary_10_1007_s10812_020_00997_1
crossref_primary_10_1117_1_3130324
crossref_primary_10_1039_c3an00309d
crossref_primary_10_1366_000370208786822179
crossref_primary_10_1002_jrs_4338
crossref_primary_10_4236_ajac_2012_32023
Cites_doi 10.1117/1.1827605
10.1117/1.1805559
10.1007/s00223-002-1039-0
10.1016/j.bone.2004.09.013
10.1366/000370206776023340
10.1366/0003702011954053
10.1016/S8756-3282(99)00163-5
10.1117/1.1908057
10.1366/000370203322554509
10.1359/JBMR.050710
10.1366/0003702981945138
10.1039/b304905a
10.1016/j.berh.2005.07.004
10.1366/000370204774103426
10.1117/12.531517
10.1364/AO.35.001654
10.1016/S8756-3282(02)00815-3
10.1016/j.vibspec.2005.02.012
10.1073/pnas.0403534101
10.1016/S8756-3282(00)00268-4
10.1016/j.biomaterials.2004.05.024
10.1366/0003702053641450
10.1016/S0736-0266(01)00152-8
10.1366/000370202760249774
10.1117/1.1924668
10.1002/jrs.892
10.1366/0003702021954647
10.1117/12.460798
10.1364/OPEX.13.002564
10.1021/ac9913560
10.1016/0021-9290(96)00020-6
10.1366/0003702981943428
ContentType Journal Article
Copyright 2006 Elsevier B.V.
Copyright_xml – notice: 2006 Elsevier B.V.
DBID 6I.
AAFTH
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1016/j.bbamem.2006.02.022
DatabaseName ScienceDirect Open Access Titles
Elsevier:ScienceDirect:Open Access
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList
MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
Biology
EISSN 1879-2642
EndPage 873
ExternalDocumentID 10_1016_j_bbamem_2006_02_022
16584709
S0005273606000745
Genre Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIAMS NIH HHS
  grantid: R01 AR05010
GroupedDBID ---
--K
--M
.~1
0R~
1B1
1RT
1~.
1~5
23N
3O-
4.4
457
4G.
53G
5GY
5RE
5VS
6I.
6J9
7-5
71M
8P~
9JM
AABNK
AACTN
AAEDT
AAEDW
AAFTH
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AAXUO
ABEFU
ABFNM
ABGSF
ABMAC
ABUDA
ABVKL
ABXDB
ABYKQ
ACDAQ
ACIUM
ACRLP
ADBBV
ADEZE
ADMUD
ADUVX
AEBSH
AEHWI
AEKER
AEXQZ
AFKWA
AFTJW
AFXIZ
AGHFR
AGUBO
AGYEJ
AHHHB
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
CS3
EBS
EFJIC
EFLBG
EJD
EO8
EO9
EP2
EP3
FDB
FEDTE
FGOYB
FIRID
FNPLU
FYGXN
G-2
G-Q
G8K
GBLVA
HLW
HVGLF
HZ~
IHE
IXB
J1W
KOM
LX3
M41
MO0
N9A
NCXOZ
O-L
O9-
OAUVE
OK1
OZT
P-8
P-9
PC.
Q38
R2-
RIG
ROL
RPZ
RSU
SBG
SCC
SDF
SDG
SDP
SES
SEW
SSU
SSZ
T5K
WH7
WUQ
XJT
XPP
~G-
-~X
.55
.GJ
AAYJJ
ABJNI
AFFNX
AI.
AKRWK
CGR
CUY
CVF
ECM
EIF
F5P
H~9
K-O
MVM
NPM
OHT
TWZ
UHS
VH1
X7M
Y6R
YYP
ZE2
ZGI
~KM
0SF
AAXKI
AAYXX
ADVLN
AFJKZ
CITATION
7X8
ID FETCH-LOGICAL-c406t-7633c9d78bc8570208a9a5a17fe0bec26236a2954b02fc258cd13659a486a5443
IEDL.DBID AIKHN
ISSN 0005-2736
0006-3002
IngestDate Sat Aug 17 01:39:42 EDT 2024
Thu Sep 26 19:35:02 EDT 2024
Sat Sep 28 07:47:20 EDT 2024
Fri Feb 23 02:23:28 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 7
Keywords Raman microspectroscopy
Bone
EMCCD
Raman imaging
Photobleaching
Language English
License http://www.elsevier.com/open-access/userlicense/1.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c406t-7633c9d78bc8570208a9a5a17fe0bec26236a2954b02fc258cd13659a486a5443
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://www.sciencedirect.com/science/article/pii/S0005273606000745
PMID 16584709
PQID 68767258
PQPubID 23479
PageCount 6
ParticipantIDs proquest_miscellaneous_68767258
crossref_primary_10_1016_j_bbamem_2006_02_022
pubmed_primary_16584709
elsevier_sciencedirect_doi_10_1016_j_bbamem_2006_02_022
PublicationCentury 2000
PublicationDate 2006-07-01
PublicationDateYYYYMMDD 2006-07-01
PublicationDate_xml – month: 07
  year: 2006
  text: 2006-07-01
  day: 01
PublicationDecade 2000
PublicationPlace Netherlands
PublicationPlace_xml – name: Netherlands
PublicationTitle Biochimica et biophysica acta
PublicationTitleAlternate Biochim Biophys Acta
PublicationYear 2006
Publisher Elsevier B.V
Publisher_xml – name: Elsevier B.V
References Nalla, Kruzic, Kinney, Ritchie (bib7) 2005; 26
Schulmerich, Finney, Fredericks, Morris (bib21) 2006; 60
Carden, Rajachar, Morris, Kohn (bib10) 2003; 72
Burr (bib6) 2002; 31
Morris, Carden, Rajachar, Kohn (bib9) 2002; 4614
Timlin, Carden, Morris, Rajachar, Kohn (bib8) 2000; 72
Chen, Shea, Morris (bib26) 2002; 56
Everall, Hahn, Matousek, Parker, Towrie (bib16) 2004; 58
Hengsberger, Ammann, Legros, Rizzoli, Zysset (bib5) 2005; 36
Janesick (bib14) 2001
Callender, Finney, Morris, Sahar, Kohn, Kozloff, Goldstein (bib12) 2005; 38
Matousek, Clark, Draper, Morris, Goodship, Everall, Towrie, Finney, Parker (bib15) 2005; 59
Hoffler, Moore, Kozloff, Zysset, Brown, Goldstein (bib2) 2000; 26
Ager III, Nalla, Breeden, Ritchie (bib34) 2005; 10
Rho, Ziopos, Currey, Pharr (bib4) 1999; 25
Christensen, Morris (bib28) 1998; 52
Morris, Finney, Rajachar, Kohn (bib11) 2004; 126
Morris, Matousek, Towrie, Parker, Goodship, Draper (bib19) 2005; 10
Crane, Morris, Ignelzi, Yu (bib33) 2005; 10
Treado, Morris (bib30) 1993
Goodwin, Sharkey (bib3) 2002; 20
Bouxsein (bib1) 2005; 19
Sarkar, Robertson, Fernandez (bib23) 2004; 101
Draper, Morris, Camacho, Matousek, Towrie, Parker, Goodship (bib20) 2005; 20
Coates, Denvir, Mchale, Thornbury, Hollywood (bib22) 2004; 9
Penel, Leroy, Brés (bib25) 1998; 52
Bewsher, Powell, Boland (bib29) 1996; 35
Morris, Goodship, Draper, Matousek, Towrie, Parker (bib18) 2004; 5321
Patwardhan, Bloch, Achilefu, Culver (bib24) 2005; 13
Everall, Hahn, Matousek, Parker, Towrie (bib17) 2001; 55
Stewart, Shea, Tarnowski, Morrisd, Wang, Franceschi, Lin, Keller (bib13) 2002; 33
Shea, Morris (bib27) 2002; 56
Widjaja, Crane, Chen, Morris, Ignelzi, McCreadie (bib31) 2003; 57
Gustafson, Martin, Gibson, Storms, Stover, Gibeling, Griffin (bib32) 1996; 29
Hoffler (10.1016/j.bbamem.2006.02.022_bib2) 2000; 26
Rho (10.1016/j.bbamem.2006.02.022_bib4) 1999; 25
Stewart (10.1016/j.bbamem.2006.02.022_bib13) 2002; 33
Gustafson (10.1016/j.bbamem.2006.02.022_bib32) 1996; 29
Morris (10.1016/j.bbamem.2006.02.022_bib19) 2005; 10
Everall (10.1016/j.bbamem.2006.02.022_bib16) 2004; 58
Patwardhan (10.1016/j.bbamem.2006.02.022_bib24) 2005; 13
Ager III (10.1016/j.bbamem.2006.02.022_bib34) 2005; 10
Matousek (10.1016/j.bbamem.2006.02.022_bib15) 2005; 59
Crane (10.1016/j.bbamem.2006.02.022_bib33) 2005; 10
Schulmerich (10.1016/j.bbamem.2006.02.022_bib21) 2006; 60
Goodwin (10.1016/j.bbamem.2006.02.022_bib3) 2002; 20
Sarkar (10.1016/j.bbamem.2006.02.022_bib23) 2004; 101
Morris (10.1016/j.bbamem.2006.02.022_bib11) 2004; 126
Draper (10.1016/j.bbamem.2006.02.022_bib20) 2005; 20
Callender (10.1016/j.bbamem.2006.02.022_bib12) 2005; 38
Coates (10.1016/j.bbamem.2006.02.022_bib22) 2004; 9
Hengsberger (10.1016/j.bbamem.2006.02.022_bib5) 2005; 36
Nalla (10.1016/j.bbamem.2006.02.022_bib7) 2005; 26
Morris (10.1016/j.bbamem.2006.02.022_bib18) 2004; 5321
Carden (10.1016/j.bbamem.2006.02.022_bib10) 2003; 72
Christensen (10.1016/j.bbamem.2006.02.022_bib28) 1998; 52
Bouxsein (10.1016/j.bbamem.2006.02.022_bib1) 2005; 19
Janesick (10.1016/j.bbamem.2006.02.022_bib14) 2001
Shea (10.1016/j.bbamem.2006.02.022_bib27) 2002; 56
Timlin (10.1016/j.bbamem.2006.02.022_bib8) 2000; 72
Morris (10.1016/j.bbamem.2006.02.022_bib9) 2002; 4614
Widjaja (10.1016/j.bbamem.2006.02.022_bib31) 2003; 57
Burr (10.1016/j.bbamem.2006.02.022_bib6) 2002; 31
Bewsher (10.1016/j.bbamem.2006.02.022_bib29) 1996; 35
Everall (10.1016/j.bbamem.2006.02.022_bib17) 2001; 55
Chen (10.1016/j.bbamem.2006.02.022_bib26) 2002; 56
Treado (10.1016/j.bbamem.2006.02.022_bib30) 1993
Penel (10.1016/j.bbamem.2006.02.022_bib25) 1998; 52
References_xml – volume: 55
  start-page: 1701
  year: 2001
  end-page: 1708
  ident: bib17
  article-title: Picosecond time-resolved Raman spectroscopy of solids: capabilities and limitations for fluorescence rejection and the influence of diffuse reflectance
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Towrie
– volume: 10
  start-page: 031119
  year: 2005
  end-page: 031128
  ident: bib33
  article-title: Raman imaging demonstrates FGF2-induced craniosynostosis in mouse calvaria
  publication-title: J. Biomed. Opt.
  contributor:
    fullname: Yu
– year: 2001
  ident: bib14
  article-title: Scientific charge coupled devices
  contributor:
    fullname: Janesick
– volume: 56
  start-page: 1035
  year: 2002
  end-page: 1037
  ident: bib26
  article-title: Effect of hydrogen peroxide bleaching on bone mineral/matrix ratio
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Morris
– volume: 5321
  start-page: 164
  year: 2004
  end-page: 169
  ident: bib18
  article-title: Kerr-gated picosecond Raman spectroscopy and Raman photon migration of equine bone tissue with 400-nm excitation
  publication-title: Proc. SPIE
  contributor:
    fullname: Parker
– volume: 101
  start-page: 12882
  year: 2004
  end-page: 12886
  ident: bib23
  article-title: Simultaneous atomic force microscopy and fluorescence measurements of protein unfolding using a calibrated evanescent wave
  publication-title: Proc. Natl. Acad. Sci.
  contributor:
    fullname: Fernandez
– volume: 20
  start-page: 600
  year: 2002
  end-page: 606
  ident: bib3
  article-title: Material properties of interstitial lamellae reflect local strain environments
  publication-title: J. Orthopaedic. Res.
  contributor:
    fullname: Sharkey
– volume: 52
  start-page: 1145
  year: 1998
  end-page: 1147
  ident: bib28
  article-title: Hyperspectral Raman microscopic imaging using Powell lens line illumination
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Morris
– volume: 25
  start-page: 295
  year: 1999
  end-page: 300
  ident: bib4
  article-title: Variations in the individual thick lamellar properties within osteons by nanoindendation
  publication-title: Bone
  contributor:
    fullname: Pharr
– volume: 10
  start-page: 034012
  year: 2005
  end-page: 034018
  ident: bib34
  article-title: Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone
  publication-title: J. Biomed. Opt.
  contributor:
    fullname: Ritchie
– volume: 10
  start-page: 014014
  year: 2005
  ident: bib19
  article-title: Kerr-gated time-resolved Raman spectroscopy of equine cortical bone tissue
  publication-title: J. Biomed. Opt.
  contributor:
    fullname: Draper
– volume: 13
  start-page: 2564
  year: 2005
  end-page: 2577
  ident: bib24
  article-title: Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice
  publication-title: Opt. Express
  contributor:
    fullname: Culver
– volume: 31
  start-page: 8
  year: 2002
  end-page: 11
  ident: bib6
  article-title: The contribution of the organic matrix to bone's material properties
  publication-title: Bone
  contributor:
    fullname: Burr
– volume: 52
  start-page: 312
  year: 1998
  end-page: 313
  ident: bib25
  article-title: New preparation method of bone samples for Raman microspectroscopy
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Brés
– volume: 33
  start-page: 536
  year: 2002
  end-page: 543
  ident: bib13
  article-title: Trends in early mineralization of murine calvarial osteoblastic cultures: a Raman microscopic study
  publication-title: J. Raman Spectrosc.
  contributor:
    fullname: Keller
– volume: 58
  start-page: 591
  year: 2004
  end-page: 597
  ident: bib16
  article-title: Photon migration in Raman spectroscopy
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Towrie
– volume: 26
  start-page: 2183
  year: 2005
  end-page: 2195
  ident: bib7
  article-title: Aspects of in vitro fatigue in human cortical bone; time and cycle dependent crack growth
  publication-title: Biomaterials
  contributor:
    fullname: Ritchie
– volume: 72
  start-page: 2229
  year: 2000
  end-page: 2236
  ident: bib8
  article-title: Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone
  publication-title: Anal. Chem.
  contributor:
    fullname: Kohn
– volume: 36
  start-page: 134
  year: 2005
  end-page: 141
  ident: bib5
  article-title: Intrinsic bone tissue properties in adult rat vertebrae: modulation by dietary protein
  publication-title: Bone
  contributor:
    fullname: Zysset
– year: 1993
  ident: bib30
  article-title: Infrared and Raman spectroscopic imaging
  publication-title: Spectroscopic imaging of the chemical state
  contributor:
    fullname: Morris
– volume: 57
  start-page: 1353
  year: 2003
  end-page: 1362
  ident: bib31
  article-title: Band-Target Entropy Minimization (BTEM) Applied to Hyperspectral Raman Image Data
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: McCreadie
– volume: 9
  start-page: 1244
  year: 2004
  end-page: 1252
  ident: bib22
  article-title: Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution
  publication-title: J. Biomed. Opt.
  contributor:
    fullname: Hollywood
– volume: 56
  start-page: 182
  year: 2002
  end-page: 186
  ident: bib27
  article-title: Bone tissue fluorescence reduction for visible laser Raman spectroscopy
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Morris
– volume: 60
  start-page: 109
  year: 2006
  end-page: 114
  ident: bib21
  article-title: Subsurface Raman spectroscopy and mapping using a globally illuminated non-confocal fiber optic array probe in the presence of Raman photon migration
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Morris
– volume: 38
  start-page: 101
  year: 2005
  end-page: 105
  ident: bib12
  article-title: Dynamic mechanical testing system for Raman microscopy of bone tissue specimens
  publication-title: Vib. Spectrosc.
  contributor:
    fullname: Goldstein
– volume: 19
  start-page: 897
  year: 2005
  end-page: 911
  ident: bib1
  article-title: Determinants of skeletal fragility
  publication-title: Best Pract. Res., Clin. Rheumatol.
  contributor:
    fullname: Bouxsein
– volume: 59
  start-page: 393
  year: 2005
  end-page: 400
  ident: bib15
  article-title: Subsurface probing in diffusely scattering media using spatially offset Raman spectroscopy
  publication-title: Appl. Spectrosc.
  contributor:
    fullname: Parker
– volume: 20
  start-page: 1968
  year: 2005
  end-page: 1972
  ident: bib20
  article-title: Novel assessment of bone using time-resolved transcutaneous Raman spectroscopy
  publication-title: J. Bone Miner Res.
  contributor:
    fullname: Goodship
– volume: 72
  start-page: 166
  year: 2003
  end-page: 175
  ident: bib10
  article-title: Ultrastructural changes accompanying the mechanical deformation of bone tissue: a Raman imaging study
  publication-title: Calcif. Tissue Int.
  contributor:
    fullname: Kohn
– volume: 126
  start-page: 159
  year: 2004
  end-page: 168
  ident: bib11
  article-title: Bone tissue ultrastructural response to elastic deformation probed by Raman spectroscopy
  publication-title: Faraday discuss.
  contributor:
    fullname: Kohn
– volume: 26
  start-page: 603
  year: 2000
  end-page: 609
  ident: bib2
  article-title: Heterogeneity of bone lamellar-level elastic moduli
  publication-title: Bone
  contributor:
    fullname: Goldstein
– volume: 4614
  start-page: 47
  year: 2002
  end-page: 54
  ident: bib9
  article-title: Effects of applied load on bone tissue as observed by Raman spectroscopy
  publication-title: Proc. SPIE
  contributor:
    fullname: Kohn
– volume: 35
  start-page: 1654
  year: 1996
  end-page: 1958
  ident: bib29
  article-title: Design of single element laser beam shape projectors
  publication-title: Appl. Opt.
  contributor:
    fullname: Boland
– volume: 29
  start-page: 1191
  year: 1996
  end-page: 1194
  ident: bib32
  article-title: Calcium buffering is required to maintain bone stiffness in saline solution
  publication-title: J. Biomech.
  contributor:
    fullname: Griffin
– volume: 10
  start-page: 014014
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib19
  article-title: Kerr-gated time-resolved Raman spectroscopy of equine cortical bone tissue
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.1827605
  contributor:
    fullname: Morris
– volume: 9
  start-page: 1244
  year: 2004
  ident: 10.1016/j.bbamem.2006.02.022_bib22
  article-title: Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.1805559
  contributor:
    fullname: Coates
– volume: 72
  start-page: 166
  year: 2003
  ident: 10.1016/j.bbamem.2006.02.022_bib10
  article-title: Ultrastructural changes accompanying the mechanical deformation of bone tissue: a Raman imaging study
  publication-title: Calcif. Tissue Int.
  doi: 10.1007/s00223-002-1039-0
  contributor:
    fullname: Carden
– volume: 36
  start-page: 134
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib5
  article-title: Intrinsic bone tissue properties in adult rat vertebrae: modulation by dietary protein
  publication-title: Bone
  doi: 10.1016/j.bone.2004.09.013
  contributor:
    fullname: Hengsberger
– volume: 60
  start-page: 109
  year: 2006
  ident: 10.1016/j.bbamem.2006.02.022_bib21
  article-title: Subsurface Raman spectroscopy and mapping using a globally illuminated non-confocal fiber optic array probe in the presence of Raman photon migration
  publication-title: Appl. Spectrosc.
  doi: 10.1366/000370206776023340
  contributor:
    fullname: Schulmerich
– volume: 55
  start-page: 1701
  year: 2001
  ident: 10.1016/j.bbamem.2006.02.022_bib17
  article-title: Picosecond time-resolved Raman spectroscopy of solids: capabilities and limitations for fluorescence rejection and the influence of diffuse reflectance
  publication-title: Appl. Spectrosc.
  doi: 10.1366/0003702011954053
  contributor:
    fullname: Everall
– volume: 25
  start-page: 295
  year: 1999
  ident: 10.1016/j.bbamem.2006.02.022_bib4
  article-title: Variations in the individual thick lamellar properties within osteons by nanoindendation
  publication-title: Bone
  doi: 10.1016/S8756-3282(99)00163-5
  contributor:
    fullname: Rho
– volume: 10
  start-page: 031119
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib33
  article-title: Raman imaging demonstrates FGF2-induced craniosynostosis in mouse calvaria
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.1908057
  contributor:
    fullname: Crane
– volume: 57
  start-page: 1353
  year: 2003
  ident: 10.1016/j.bbamem.2006.02.022_bib31
  article-title: Band-Target Entropy Minimization (BTEM) Applied to Hyperspectral Raman Image Data
  publication-title: Appl. Spectrosc.
  doi: 10.1366/000370203322554509
  contributor:
    fullname: Widjaja
– volume: 20
  start-page: 1968
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib20
  article-title: Novel assessment of bone using time-resolved transcutaneous Raman spectroscopy
  publication-title: J. Bone Miner Res.
  doi: 10.1359/JBMR.050710
  contributor:
    fullname: Draper
– volume: 52
  start-page: 1145
  year: 1998
  ident: 10.1016/j.bbamem.2006.02.022_bib28
  article-title: Hyperspectral Raman microscopic imaging using Powell lens line illumination
  publication-title: Appl. Spectrosc.
  doi: 10.1366/0003702981945138
  contributor:
    fullname: Christensen
– volume: 126
  start-page: 159
  year: 2004
  ident: 10.1016/j.bbamem.2006.02.022_bib11
  article-title: Bone tissue ultrastructural response to elastic deformation probed by Raman spectroscopy
  publication-title: Faraday discuss.
  doi: 10.1039/b304905a
  contributor:
    fullname: Morris
– volume: 19
  start-page: 897
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib1
  article-title: Determinants of skeletal fragility
  publication-title: Best Pract. Res., Clin. Rheumatol.
  doi: 10.1016/j.berh.2005.07.004
  contributor:
    fullname: Bouxsein
– volume: 58
  start-page: 591
  year: 2004
  ident: 10.1016/j.bbamem.2006.02.022_bib16
  article-title: Photon migration in Raman spectroscopy
  publication-title: Appl. Spectrosc.
  doi: 10.1366/000370204774103426
  contributor:
    fullname: Everall
– volume: 5321
  start-page: 164
  year: 2004
  ident: 10.1016/j.bbamem.2006.02.022_bib18
  article-title: Kerr-gated picosecond Raman spectroscopy and Raman photon migration of equine bone tissue with 400-nm excitation
  publication-title: Proc. SPIE
  doi: 10.1117/12.531517
  contributor:
    fullname: Morris
– year: 1993
  ident: 10.1016/j.bbamem.2006.02.022_bib30
  article-title: Infrared and Raman spectroscopic imaging
  contributor:
    fullname: Treado
– volume: 35
  start-page: 1654
  year: 1996
  ident: 10.1016/j.bbamem.2006.02.022_bib29
  article-title: Design of single element laser beam shape projectors
  publication-title: Appl. Opt.
  doi: 10.1364/AO.35.001654
  contributor:
    fullname: Bewsher
– volume: 31
  start-page: 8
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib6
  article-title: The contribution of the organic matrix to bone's material properties
  publication-title: Bone
  doi: 10.1016/S8756-3282(02)00815-3
  contributor:
    fullname: Burr
– volume: 38
  start-page: 101
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib12
  article-title: Dynamic mechanical testing system for Raman microscopy of bone tissue specimens
  publication-title: Vib. Spectrosc.
  doi: 10.1016/j.vibspec.2005.02.012
  contributor:
    fullname: Callender
– volume: 101
  start-page: 12882
  year: 2004
  ident: 10.1016/j.bbamem.2006.02.022_bib23
  article-title: Simultaneous atomic force microscopy and fluorescence measurements of protein unfolding using a calibrated evanescent wave
  publication-title: Proc. Natl. Acad. Sci.
  doi: 10.1073/pnas.0403534101
  contributor:
    fullname: Sarkar
– volume: 26
  start-page: 603
  year: 2000
  ident: 10.1016/j.bbamem.2006.02.022_bib2
  article-title: Heterogeneity of bone lamellar-level elastic moduli
  publication-title: Bone
  doi: 10.1016/S8756-3282(00)00268-4
  contributor:
    fullname: Hoffler
– volume: 26
  start-page: 2183
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib7
  article-title: Aspects of in vitro fatigue in human cortical bone; time and cycle dependent crack growth
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2004.05.024
  contributor:
    fullname: Nalla
– volume: 59
  start-page: 393
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib15
  article-title: Subsurface probing in diffusely scattering media using spatially offset Raman spectroscopy
  publication-title: Appl. Spectrosc.
  doi: 10.1366/0003702053641450
  contributor:
    fullname: Matousek
– year: 2001
  ident: 10.1016/j.bbamem.2006.02.022_bib14
  contributor:
    fullname: Janesick
– volume: 20
  start-page: 600
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib3
  article-title: Material properties of interstitial lamellae reflect local strain environments
  publication-title: J. Orthopaedic. Res.
  doi: 10.1016/S0736-0266(01)00152-8
  contributor:
    fullname: Goodwin
– volume: 56
  start-page: 1035
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib26
  article-title: Effect of hydrogen peroxide bleaching on bone mineral/matrix ratio
  publication-title: Appl. Spectrosc.
  doi: 10.1366/000370202760249774
  contributor:
    fullname: Chen
– volume: 10
  start-page: 034012
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib34
  article-title: Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.1924668
  contributor:
    fullname: Ager III
– volume: 33
  start-page: 536
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib13
  article-title: Trends in early mineralization of murine calvarial osteoblastic cultures: a Raman microscopic study
  publication-title: J. Raman Spectrosc.
  doi: 10.1002/jrs.892
  contributor:
    fullname: Stewart
– volume: 56
  start-page: 182
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib27
  article-title: Bone tissue fluorescence reduction for visible laser Raman spectroscopy
  publication-title: Appl. Spectrosc.
  doi: 10.1366/0003702021954647
  contributor:
    fullname: Shea
– volume: 4614
  start-page: 47
  year: 2002
  ident: 10.1016/j.bbamem.2006.02.022_bib9
  article-title: Effects of applied load on bone tissue as observed by Raman spectroscopy
  publication-title: Proc. SPIE
  doi: 10.1117/12.460798
  contributor:
    fullname: Morris
– volume: 13
  start-page: 2564
  year: 2005
  ident: 10.1016/j.bbamem.2006.02.022_bib24
  article-title: Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice
  publication-title: Opt. Express
  doi: 10.1364/OPEX.13.002564
  contributor:
    fullname: Patwardhan
– volume: 72
  start-page: 2229
  year: 2000
  ident: 10.1016/j.bbamem.2006.02.022_bib8
  article-title: Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone
  publication-title: Anal. Chem.
  doi: 10.1021/ac9913560
  contributor:
    fullname: Timlin
– volume: 29
  start-page: 1191
  year: 1996
  ident: 10.1016/j.bbamem.2006.02.022_bib32
  article-title: Calcium buffering is required to maintain bone stiffness in saline solution
  publication-title: J. Biomech.
  doi: 10.1016/0021-9290(96)00020-6
  contributor:
    fullname: Gustafson
– volume: 52
  start-page: 312
  year: 1998
  ident: 10.1016/j.bbamem.2006.02.022_bib25
  article-title: New preparation method of bone samples for Raman microspectroscopy
  publication-title: Appl. Spectrosc.
  doi: 10.1366/0003702981943428
  contributor:
    fullname: Penel
SSID ssj0016425
ssj0025309
Score 2.1052985
Snippet Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and...
SourceID proquest
crossref
pubmed
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 868
SubjectTerms Animals
Bone
Bone and Bones - chemistry
Cattle
Diagnostic Imaging - methods
EMCCD
Fluorescence
Fluorescence Recovery After Photobleaching
Lasers
Minerals - analysis
Photobleaching
Raman imaging
Raman microspectroscopy
Spectrum Analysis, Raman - methods
Title Is photobleaching necessary for Raman imaging of bone tissue using a green laser?
URI https://dx.doi.org/10.1016/j.bbamem.2006.02.022
https://www.ncbi.nlm.nih.gov/pubmed/16584709
https://search.proquest.com/docview/68767258
Volume 1758
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fS-NAEB60cpwvop6n9UfdB19zbjabH_skbVFaeycoevYtbJKNVmhaanzwxb_dmWyi-CCCsBBICNnMhJmPzDfzARz5XmZy37hOTsMqZe4ZRykZOonnUYePMKmmfud_F8HgRp6P_fES9JteGKJV1rHfxvQqWtdnjmtrHs8nE-rx5TQ9DBF4lQj9ZVjBdCRlC1a6w9Hg4q2YgBDbChlwar-iauX-O80rSfTUTOuqhMAlPstQnyHQKhOdrcNaDSFZ1-5yA5ZMsQk_rKjk8yb87Dcabr_gcvjI5vezkkRjLGuSFYYaA_TimSFcZVd6qgs2mVZaRWyWs2RWGFZW3mDEib9jmt0RN4chzDaLky24OTu97g-cWkTBSTFXlw7GDy9VWRglKc2yFzzSSvvaDXPD0X8C4U-gqdiXcJGnwo_SjJhvSsso0DQc7ze0Cnz0DrA8y7xQKim5VtKNtHYz36sGKoaKJ17QBqcxXDy3szLihkT2EFtDk-xlEHOBS7QhbKwbf_B5jOH8izsPG2fEaFGqcejCzJ4e4wCje4hv0YZt66P3nRDWCrna_fZT92DV_oAhsu4-tMrFkzlASFImHVj-8-J28MPr_R_97dQfIB1HV7cjvDoc914BugrhZQ
link.rule.ids 315,786,790,3525,4521,24144,27600,27955,27956,45618,45696,45712,45907
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEB58IHoR31ar7sFrcLvZPPYkWpT6BEWht2WTbLRC01LTQ_-9M9lE8VAEYU-BsJuZMPMl8818AKeBn9k8sB0vp2GVMvetp5SMvMT3qcNH2NRQv_PDY9h7lbf9oL8A3aYXhmiVdex3Mb2K1vWVs9qaZ-PBgHp8OU0PQwReJcJgEZZlgN961MXXv_wuJSDAdjIGnJqvqFbZ_iF5JYkZ2mFdkxC4xLz8NA9_VnnoegPWawDJLtwZN2HBFluw4iQlZ1uw2m0U3Lbh6eaTjd9HJUnGOM4kKyy1BZjJjCFYZc9maAo2GFZKRWyUs2RUWFZWvmDEiH9jhr0RM4chyLaT8x14vb566fa8WkLBSzFTlx5GDz9VWRQnKU2yFzw2ygSmE-WWo_cEgp_QUKkv4SJPRRCnGfHelJFxaGg03i4sFbj1PrA8y_xIKim5UbITG9PJAr8apxgpnvhhC7zGcHrsJmXohkL2oZ2hSfQy1FzgEi2IGuvqXx7XGMz_uPOkcYZGi1KFwxR2NP3UIcb2CJ-iBXvORz8nIaQVcXXw711PYLX38nCv728e7w5hzf2KIdpuG5bKydQeITgpk-Pq5fsCi0veDQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Is+photobleaching+necessary+for+Raman+imaging+of+bone+tissue+using+a+green+laser%3F&rft.jtitle=Biochimica+et+biophysica+acta.+Biomembranes&rft.au=Golcuk%2C+Kurtulus&rft.au=Mandair%2C+Gurjit+S.&rft.au=Callender%2C+Andrew+F.&rft.au=Sahar%2C+Nadder&rft.date=2006-07-01&rft.issn=0005-2736&rft.volume=1758&rft.issue=7&rft.spage=868&rft.epage=873&rft_id=info:doi/10.1016%2Fj.bbamem.2006.02.022&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_bbamem_2006_02_022
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0005-2736&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0005-2736&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0005-2736&client=summon