Brevibacterium anseongense sp. nov., isolated from soil of ginseng field

• Published in: The journal of microbiology Vol. 56; no. 10; pp. 706 - 712
• Main Authors: Jung, Mi-Seon, Quan, Xiao-Tian, Siddiqi, Muhammad Zubair, Liu, Qingzhen, Kim, Sang Yong, Wee, Ji-Hyang, Im, Wan Taek
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.10.2018; Springer Nature B.V; 한국미생물학회
• Subjects: Bacterial Typing Techniques; Base Composition; Biochemical characteristics; Biochemistry; Biomedical and Life Sciences; Brevibacterium; Brevibacterium - classification; Brevibacterium - genetics; Brevibacterium - isolation & purification; Cardiolipin; Deoxyribonucleic acid; Diaminopimelic Acid - analysis; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Ginseng; Life Sciences; Lipids; Microbial Systematics and Evolutionary Microbiology; Microbiology; New species; Nucleotide sequence; Panax - microbiology; Peptidoglycans; Phosphatidylglycerol; Phosphatidylglycerols - analysis; Phylogeny; Quinones; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Soil; Soil Microbiology; 생물학; Republic of Korea; 16S rRNA gene sequence; polyphasic taxonomy; Brevibacterium anseongensis
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-018-8181-5

Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped bacterium, designated as Gsoil 188 T , was isolated from the soil of a ginseng field in Pocheon, South Korea. A phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to B. epidermidis NBRC 14811 T (98.4%), B. sediminis FXJ8.269 T (98.2%), B. avium NCFB 3055 T (98.1%), and B. oceani BBH7 T (98.1%), while it shared less than 98.1% identity with the other species of this genus. The DNA G + C content was 68.1 mol%. The predominant quinone was MK-8(H 2 ). The major fatty acids were anteiso-C 15:0 and anteiso-C 17:0 . The cell wall peptidoglycan of strain Gsoil 188 T contained meso -diaminopimelic acid. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and an unidentified aminolipid. The physiological and biochemical characteristics, low DNA-DNA relatedness values, and taxonomic analysis allowed the differentiation of strain Gsoil 188 T from the other recognized species of the genus Brevibacterium . Therefore, strain Gsoil 188 T represents a novel species of the genus Brevibacterium , for which the name Brevibacterium anseongense sp. nov. is proposed, with the type strain Gsoil 188 T (= KACC 19439 T = LMG 30331 T ).