Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongy...

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Published inParasitology Vol. 144; no. 2; pp. 124 - 130
Main Authors CORRAL, M. A., PAULA, F. M., MEISEL, D. M. C. L., CASTILHO, V. L. P., GONÇALVES, E. M. N., LEVY, D., BYDLOWSKI, S. P., CHIEFFI, P. P., CASTRO-BORGES, W., GRYSCHEK, R. C. B.
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.02.2017
Subjects
Animals
Antigens, Helminth
Biomarkers - blood
Enzyme-Linked Immunosorbent Assay
Helminth Proteins - immunology
Humans
Larvae
Mass spectrometry
Nematoda
Sensitivity and Specificity
Strongyloides
Strongyloides - immunology
Strongyloidiasis - blood
Strongyloidiasis - diagnosis
Strongyloidiasis - immunology
immunodiagnostic
actin
antigens
Strongyloides venezuelensis
galectin
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Summary:Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris–HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30–40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.
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ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182016001645