Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2

• Published in: Antibiotics (Basel) Vol. 11; no. 11; p. 1524
• Main Authors: Kang, Min-Gyun, Khan, Fazlurrahman, Jo, Du-Min, Oh, DoKyung, Tabassum, Nazia, Kim, Young-Mog
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.11.2022; MDPI
• Subjects: Absorption spectra; antibiofilm; Antimicrobial activity; Antimicrobial agents; Antimicrobial resistance; Bacteria; Biocompatibility; Biofilms; Candida albicans; Drug resistance; E coli; Gene sequencing; Gold; Gram-negative bacteria; Gram-positive bacteria; Kimchi; Lactic acid; Lactic acid bacteria; Leuconostoc; Leuconostoc sp. strain C2; Metal oxides; Microorganisms; Nanoparticles; Pathogens; Probiotics; Pseudomonas aeruginosa; rRNA 16S; Staphylococcus aureus; supernatant; Synthesis; Virulence; Zeta potential; Zinc oxide; Zinc oxides
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics11111524

The rapid emergence of antimicrobial resistance (AMR) among bacterial pathogens results in antimicrobial treatment failure and the high mortality rate associated with AMR. The application of nanoparticles synthesized from probiotics will be widely accepted due to their efficacy and biocompatibility in treating microbial infections in humans. The current work sought to isolate and identify lactic acid bacteria (LAB) from Kimchi. Based on 16S rRNA gene sequencing, the LAB isolate C2 was identified as a member of the genus Leuconostoc. The obtained supernatant from Leuconostoc sp. strain C2 was employed for the green synthesis of metal (AuNPs) and metal oxide (ZnONPs) nanoparticles. UV–vis absorption spectra, FTIR analysis, XRD, DLS, FE-TEM, and EDS mapping were used to fully characterize these C2-AuNPs and C2-ZnONPs. The C2-AuNPs were found to be spherical in shape, with a size of 47.77 ± 5.7 nm and zeta potential of −19.35 ± 0.67 mV. The C2-ZnONPs were observed to be rod-shaped and 173.77 ± 14.53 nm in size. The C2-ZnONPs zeta potential was determined to be 26.62 ± 0.35 mV. The C2-AuNPs and C2-ZnONPs were shown to have antimicrobial activity against different pathogens. Furthermore, these nanoparticles inhibited the growth of Candida albicans. The antibiofilm and antivirulence properties of these NPs against Pseudomonas aeruginosa and Staphylococcus aureus were thoroughly investigated. C2-AuNPs were reported to be antibiofilm and antivirulence against P. aeruginosa, whereas C2-ZnONPs were antibiofilm and antivirulence against both P. aeruginosa and S. aureus. Furthermore, these nanoparticles disrupted the preformed mature biofilm of P. aeruginosa and S. aureus. The inhibitory impact was discovered to be concentration-dependent. The current research demonstrated that C2-AuNPs and C2-ZnONPs exhibited potential inhibitory effects on the biofilm and virulence features of bacterial pathogens. Further studies are needed to unravel the molecular mechanism behind biofilm inhibition and virulence attenuation.