Long-wavelength fluorescent boronate probes for the detection and intracellular imaging of peroxynitrite

Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria...

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Published inChemical communications (Cambridge, England) Vol. 53; no. 95; pp. 12822 - 12825
Main Authors Sedgwick, Adam C, Han, Hai-Hao, Gardiner, Jordan E, Bull, Steven D, He, Xiao-Peng, James, Tony D
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 28.11.2017
Royal Society of Chemistry
Subjects
aqueous solutions
chemical compounds
chemical reactions
Chemistry
Chemistry, Multidisciplinary
fluorescence
fluorescent dyes
Fluorescent indicators
image analysis
Mitochondria
Physical Sciences
Science & Technology
solubility
DUAL-CHANNEL PROBE
OXIDATIVE STRESS
SUPEROXIDE
FAR-RED
CHROMOPHORES
IN-VIVO
NITRIC-OXIDE
HYDROGEN-PEROXIDE
LIVING CELLS
NITRATION
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Abstract Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459). Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ).
AbstractList Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).
Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).
Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).
Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459). Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ).
Author Bull, Steven D
Sedgwick, Adam C
Han, Hai-Hao
James, Tony D
Gardiner, Jordan E
He, Xiao-Peng
AuthorAffiliation Department of Chemistry
University of Bath
East China University of Science and Technology
Key Laboratory for Advanced Materials & Feringa Nobel Prize Scientist Joint Research Center
AuthorAffiliation_xml – sequence: 0
  name: Key Laboratory for Advanced Materials & Feringa Nobel Prize Scientist Joint Research Center
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  name: Department of Chemistry
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Author_xml – sequence: 1
  givenname: Adam C
  surname: Sedgwick
  fullname: Sedgwick, Adam C
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  givenname: Hai-Hao
  surname: Han
  fullname: Han, Hai-Hao
– sequence: 3
  givenname: Jordan E
  surname: Gardiner
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  givenname: Steven D
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  fullname: Bull, Steven D
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  givenname: Xiao-Peng
  surname: He
  fullname: He, Xiao-Peng
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  surname: James
  fullname: James, Tony D
BackLink https://www.ncbi.nlm.nih.gov/pubmed/29143045$$D View this record in MEDLINE/PubMed
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Keywords DUAL-CHANNEL PROBE
OXIDATIVE STRESS
SUPEROXIDE
FAR-RED
CHROMOPHORES
IN-VIVO
NITRIC-OXIDE
HYDROGEN-PEROXIDE
LIVING CELLS
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Snippet Two boronate fluorescent probes have been developed for the detection of peroxynitrite ( TCFB1 and TCFB2 ). TCFB1 was shown to have a low sensitvity towards...
Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards...
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SubjectTerms aqueous solutions
chemical compounds
chemical reactions
Chemistry
Chemistry, Multidisciplinary
fluorescence
fluorescent dyes
Fluorescent indicators
image analysis
Mitochondria
Physical Sciences
Science & Technology
solubility
Title Long-wavelength fluorescent boronate probes for the detection and intracellular imaging of peroxynitrite
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