A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice by in situ bioluminescence imaging

• Published in: RSC advances Vol. 10; no. 22; pp. 13029 - 13036
• Main Authors: Zhao, Ning, Liu, Jing-Min, Liu, Shuang, Ji, Xue-Meng, Lv, Huan, Hu, Yao-Zhong, Wang, Zhi-Hao, Lv, Shi-Wen, Li, Chun-Yang, Wang, Shuo
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 31.03.2020; The Royal Society of Chemistry
• Subjects: Bacteria; Bioluminescence; Chemistry; Colon; E coli; Food; Food irradiation; Medical imaging; Nutrition; Probiotics; Real time
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d0ra01283a

Food-borne bacteria have received increasing attention due to their great impact on human health. Bioimaging makes it possible to monitor bacteria inside the living body in real time and . Nano-luciferase (NLuc) as a new member of the luciferase family exhibits superior properties than the commonly used luciferases, including small size, high stability and improved luminescence. Herein, NLuc, CBRLuc and FLuc were well expressed in varied food-borne bacteria. Results showed that the signal intensity of -NLuc was about 41 times higher than -CBRLuc, -NLuc was nearly 227 times that of -FLuc Moreover, NLuc was applied to trace and through the whole body and separated digestive tract imaging, as well as the feces bacterium counting and probing. The persistence of bioluminescent strains was predominantly localized in colon and cecum of mice after oral administration. The NLuc system showed its incomparable superiority, especially in the application of intestinal imaging and the universality for food-borne bacteria. We demonstrated that the NLuc system was a brilliant alternative for specific application of food-borne bacteria , aiming to collect more accurate and real-time information of food-borne bacteria from the living body for further investigation of their damage mechanism and nutrition effect.