Analysis of transcriptomic, lipidomic and phospho-kinase profiles reveals the effects of chlorogenic acid on 3T3-L1 preadipocytes differentiation

• Published in: Journal of functional foods Vol. 110; p. 105828
• Main Authors: Lin, Shaoling, Lin, Zhongjing, Zhang, Yifei, Zheng, Baodong, Tan, Bee K., Zhang, Yi, Hu, Jiamiao
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2023; Elsevier
• Subjects: 3T3-L1 preadipocytes; Chlorogenic acid; Lipidomics; Phospho-kinase; Transcriptomics; 3T3-L1 preadipocytes; Lipidomics; Phospho-kinase; Chlorogenic acid; Transcriptomics
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2023.105828

[Display omitted] •CGA treatment during differentiation resulted in transcriptome changes in 3 T3-L1 cells.•CGA treatment during differentiation altered cellular lipid profile in 3 T3-L1 cells.•CGA treatment during differentiation inhibited adipogenesis-related kinases in 3 T3-L1 cells. Chlorogenic acid has been widely reported to profoundly influence preadipocyte differentiation but without consensus being achieved. In the current study, 3 T3-L1 preadipocytes were differentiated using adipogenic cocktail with or without chlorogenic acid. The influences of chlorogenic acid on the mRNA, lipid and phospho-kinase profiles were evaluated using transcriptomic analysis, lipidomic analysis and phospho-kinase array assay. The obtained results showed that chlorogenic acid treatment during 3T3-L1 cells differentiation altered a series of genes transcription, resulted in obvious changes in the contents of various lipids, and inhibited phosphorylation of several adipogenesis-related kinases. Overall, the observed changes in transcriptomic, lipidomic and phospho-kinase profiles in the current study favor the findings that chlorogenic acid may inhibit the differentiation of 3T3-L1 preadipocytes. In conclusion, the findings from this study highlighted the therapeutic potential of chlorogenic acid for the treatment of obesity.