Electrocatalytic NAD(P)H regeneration for biosynthesis

• Published in: Green chemical engineering Vol. 5; no. 1; pp. 1 - 15
• Main Authors: Li, Yaoxuan, Liu, Guanhua, Kong, Weixi, Zhang, Suoqing, Bao, Yuemei, Zhao, Hao, Wang, Lihui, Zhou, Liya, Jiang, Yanjun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.2024; KeAi Communications Co. Ltd
• Subjects: Bioelectrocatalysis; Biosynthesis; Cofactor regeneration; Electron mediator; NAD(P)H; Electron mediator; Biosynthesis; NAD(P)H; Cofactor regeneration; Bioelectrocatalysis
• ISSN: 2666-9528; 2666-9528
• DOI: 10.1016/j.gce.2023.02.001

The highly efficient chemoselectivity, stereoselectivity, and regioselectivity render enzyme catalysis an ideal pathway for the synthesis of various chemicals in broad applications. While the cofactor of an enzyme is necessary but expensive, the conversed state of the cofactor is not beneficial for the positive direction of the reaction. Cofactor regeneration using electrochemical methods has the advantages of simple operation, low cost, easy process monitoring, and easy product separation, and the electrical energy is green and sustainable. Therefore, bioelectrocatalysis has great potential in synthesis by combining electrochemical cofactor regeneration with enzymatic catalysis. In this review, we detail the mechanism of cofactor regeneration and categorize the common electron mediators and enzymes used in cofactor regeneration. The reaction type and the recent progress are summarized in electrochemically coupled enzymatic catalysis. The main challenges of such electroenzymatic catalysis are pointed out and future developments in this field are foreseen. [Display omitted] •Combining electrocatalytic cofactor regeneration and biocatalysis is promising.•The electron mediators and enzymes for NAD(P)H regeneration is introduced.•The reaction types of electrocatalysis coupled biosynthesis are introduced.•The advantages, problems, and development prospects of this field are prospected.