Effects of cyclosporine on responses of murine B cells to T cell- derived lymphokines

• Published in: The Journal of immunology (1950) Vol. 137; no. 7; pp. 2220 - 2224
• Main Authors: O'Garra, A, Warren, DJ, Holman, M, Popham, AM, Sanderson, CJ, Klaus, GG
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.10.1986; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Anti-Idiotypic - immunology; B-Lymphocytes - cytology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Biological and medical sciences; Cell Differentiation - drug effects; Cyclosporins - pharmacology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Growth Substances - pharmacology; Immunobiology; Interleukin-4; Lymphocyte Activation - drug effects; Lymphocyte Cooperation - drug effects; Lymphokines - pharmacology; Lymphokines, interleukins ( function, expression); Mice; Regulatory factors and their cellular receptors; T-Lymphocytes - immunology; B cell growth factor II; Rodentia; Activation; B-Lymphocyte; Lymphokine; B cell stimulatory factor-1; Vertebrata; Mammalia; Mouse; Animal; Immunosuppressive agent; Mechanism of action; Growth factor
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.137.7.2220

Cyclosporine (CS) inhibits the stimulation of both T and B lymphocytes by certain agents, but not by others. Here we have studied the effects of the drug on the responses of murine B cells to T cell-derived B cell growth and differentiation factors. We show that activation of resting B cells by B cell-stimulatory factor-1 (BSF-1) is resistant to CS, whereas stimulation by anti-Ig antibodies is not, which is in agreement with earlier findings. Furthermore, B cell proliferation elicited by co-stimulation with anti-Ig plus BSF-1 remains drug susceptible. In contrast, the stimulation of large (presumably preactivated) B cells by B cell growth factor II to synthesize DNA or to secrete Ig is inhibited by low concentrations of CS. These results therefore contrast with earlier findings with human B cells, and with those using T cells from various species, which showed that the responses of preactivated cells to growth factors are resistant to the drug. It thus appears that in the mouse CS can affect all stages of B cell stimulation.