Effect of hemolysis on insulin determination by the Beckman Coulter Unicell DXI 800 immunoassay analyzer

• Published in: Clinical biochemistry Vol. 43; no. 6; pp. 621 - 622
• Main Authors: Cook, Paul R., Glenn, Christine, Armston, Annie
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.04.2010; Elsevier
• Subjects: Biological and medical sciences; Blood Chemical Analysis - instrumentation; Blood Chemical Analysis - methods; Blood Preservation - adverse effects; Blood Specimen Collection - methods; Hemoglobins - analysis; Hemolysis; Hemolysis - physiology; Humans; Hypoglycemia; Immunoassay - instrumentation; Immunoassay - methods; Insulin; Insulin - analysis; Insulin - blood; Interference; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Osmolar Concentration; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Hemolysis; Hypoglycemia; Insulin; Interference; Pancreatic hormone; Measurement method; Analyzer; Quantitative analysis; Immunological method
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2010.01.002

To evaluate the effect of hemolysis on the measurement of insulin using the Beckman Coulter Unicell DXI 800 immunoassay analyzer. Insulin concentrations were measured using the Beckman Coulter Unicell DXI 800. Interference studies were conducted by mixing plasma of known insulin concentrations with hemolysates of increasing hemoglobin concentration. Hemolysates were prepared by osmotic shock. A change of > 10% from baseline results was taken as evidence of significant interference. Hemolysates mediated a concentration-dependent reduction in insulin concentration. A concentration-dependent negative bias in the measurement of insulin is associated with increasing degrees of hemolysis on the Beckman Coulter Unicell DXI 800.