Whole-Genome Sequencing of ST2 A. baumannii Causing Bloodstream Infections in COVID-19 Patients

A total of 43 A. baumannii strains, isolated from 43 patients affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and by bacterial sepsis, were analyzed by antimicrobial susceptibility testing. All strains were resistant to almost three different classes of antibiotics, includin...

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Published inAntibiotics (Basel) Vol. 11; no. 7; p. 955
Main Authors Cherubini, Sabrina, Perilli, Mariagrazia, Segatore, Bernardetta, Fazii, Paolo, Parruti, Giustino, Frattari, Antonella, Amicosante, Gianfranco, Piccirilli, Alessandra
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 15.07.2022
MDPI
Subjects
Acinetobacter baumannii
Antibiotic resistance
Antibiotics
Antiinfectives and antibacterials
Carbapenems
Cephalosporinase
Colistin
Coronaviruses
COVID-19
Drug resistance
Gene sequencing
Genomes
Insertion sequences
Multilocus sequence typing
Nosocomial infections
Pathogenicity
Pathogens
Sepsis
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Strains (organisms)
Virulence
Virulence factors
WGS
Whole genome sequencing
Italy
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Summary:A total of 43 A. baumannii strains, isolated from 43 patients affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and by bacterial sepsis, were analyzed by antimicrobial susceptibility testing. All strains were resistant to almost three different classes of antibiotics, including carbapenems and colistin. The whole-genome sequencing (WGS) of eight selected A. baumannii isolates showed the presence of different insertion sequences (ISs), such as ISAba13, ISAba26, IS26, ISVsa3, ISEc29, IS6100 and IS17, giving to A. baumannii a high ability to capture and mobilize antibiotic resistance genes. Resistance to carbapenems is mainly mediated by the presence of OXA-23, OXA-66 and OXA-82 oxacillinases belonging to OXA-51-like enzymes. The presence of AmpC cephalosporinase, ADC-25, was identified in all A. baumannii. The pathogenicity of A. baumannii was exacerbated by the presence of several virulence factors. The multi-locus sequence typing (MLST) analysis showed that all strains belong to sequence type 2 (ST) international clone.
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ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics11070955