Construction of Bioartificial Renal Tubule Assist Device In Vitro and Its Function of Transporting Sodium and Glucose

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 29; no. 4; pp. 517 - 521
• Main Author: 董兴刚 陈江华 何强 杨毅 章维
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.08.2009; Kidney Diseases Center, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
• Subjects: Animals; Biological Transport, Active; Bioreactors; Cell Line; Cells, Cultured; Epithelial Cells - cytology; Equipment Design; Glucose - metabolism; Kidney Tubules - cytology; Kidney Tubules - metabolism; Kidneys, Artificial; Medicine; Medicine & Public Health; Membranes, Artificial; RAD公司; Rats; Sodium - metabolism; 体外培养; 原子力显微镜; 生物反应器; 肾小管上皮细胞; 葡萄糖; 辅助装置; renal tubular epithelial cell line; transport; bioartificial renal tubule assist device; bioreactor; cell therapy; biore-actor
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-009-0425-5

To explore a new way of constructing bioartificial renal tubule assist device （RAD） in vitro and its function of transporting sodium （Na^＋） and glucose and to evaluate the application of atomic force microscope in the RAD construction, rat renal tubular epithelial cell line NRK-52E was cultured in vitro, seeded onto the outer surfaces of hollow fibers in a bioreactor, and then cultured for two weeks to construct RAD. Bioreactor hollow fibers without NRK-52E cells were used as control. The morphologies of attached cells were observed with scanning electron microscope, and the junctions of cells and polysulfone membrane were observed with atomic force microscope. Transportation of Na＋ and glucose was measured. Oubaine and phlorizin were used to inhibit the transporting property. The results showed that NRK-52E cells and polysulfone membrane were closely linked, as observed under atomic force microscope. After exposure to oubaine and phlorizin, transporting rates of Na^＋ and glucose were decreased significantly in the RAD group as compared with that in the control group （P〈0.01）. Furthermore, when the inhibitors were removed, transportation of Na^＋ and glucose was restored. It is concluded that a new RAD was constructed successfully in vitro, and it is able to selectively transport Na^＋ and glucose.