Lipid Composition and Fluidity of Plasma Membranes Isolated from Corn (Zea mays L.) Roots

• Published in: Archives of biochemistry and biophysics Vol. 387; no. 1; pp. 35 - 40
• Main Authors: Bohn, Matthias, Heinz, Ernst, Lüthje, Sabine
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2001
• Subjects: Cell Fractionation; Cell Membrane - chemistry; corn roots; Fatty Acids - analysis; fluorescence polarization; free sterols; glucocerebrosides; Glucosylceramides - analysis; hydroxy fatty acids; lipids; Lipids - chemistry; Membrane Fluidity; phospholipids; Phospholipids - analysis; Plant Roots - chemistry; plasma membrane; Sterols - analysis; Subcellular Fractions - chemistry; very long chain fatty acids; Zea mays; Zea mays L; lipids; hydroxy fatty acids; plasma membrane; corn roots; very long chain fatty acids; free sterols; glucocerebrosides; fluorescence polarization; phospholipids; Zea mays L
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1006/abbi.2000.2224

Although the results of lipid analyses from several plant species have been available for many years a complete characterization of the corn root plasma membrane is still lacking. The present study provides a detailed analysis of individual lipids and a characterization of the membrane fluidity of corn (Zea mays L.) root plasma membranes isolated by phase-partitioning. Phospholipids (43.9 mol%), sterols (40.8 mol%), and sphingolipids in the form of glucocerebroside (6.8 mol%) constitute the major lipid classes. Stigmasterol (19.8 mol%), campesterol (13.0 mol%), phosphatidylcholine 15.8 mol%), and phosphatidylethanolamine (14.2 mol%) represent the most ubiquitous individual lipids. Hydroxy fatty acids make up 80.9 mol% and very long chain fatty acids are almost 78% of fatty acids in glucocerebroside. Hydroxy arachidic acid (20:0 h) and hydroxy lignoceric acid (24:0 h) are most prominent and glucocerebroside from corn root plasma membranes contains virtually no unsaturated fatty acids. Among the phospholipids only phosphatidylserine displayed a high proportion of very long chain fatty acids (e.g., behenic and lignoceric acid). Membrane fluidity was estimated by fluorescence anisotropy. Due to the high sterol content the plasma membrane of corn roots is relatively rigid.