Eckol protects against acute experimental colitis in mice: Possible involvement of Reg3g

• Published in: Journal of functional foods Vol. 73; p. 104088
• Main Authors: Zhu, Yujie, Guo, Jie, Hu, Xianmin, Liu, Juan, Li, Shulan, Wang, Jun
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2020; Elsevier
• Subjects: Colitis; Eckol; Marine brown algae; Phlorotannin; Reg3g; Eckol; Phlorotannin; Reg3g; Colitis; Marine brown algae
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2020.104088

[Display omitted] •Protective effect of eckol on the DSS-induced acute colitis development.•Anti-inflammatory and anti-apoptotic activities of eckol in colitic mice.•Antimicrobial and immunoregulatory activities of eckol in colitic mice.•Possible involvement of Reg3g in eckol-mediated protection in colitis. The anti-inflammatory activity of eckol, a phlorotannin constituent of marine brown algae, has been widely demonstrated. Here, we found eckol at doses of 0.5–1.0 mg/kg protected against development of dextran sulfate sodium (DSS)-induced acute experimental colitis in mice. DSS-elevated colonic tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 levels as well as nuclear factor kappa B(NF-κB)p65 and Toll like receptor (TLR)-4 expression were significantly suppressed, the colonic IL-10 level was enhanced in the eckol-treated mice. TUNEL-positive cells and Caspase-9 expression were downregulated in eckol-treated colons compared to DSS controls. Eckol inhibited the growth of potential gut pathogens and increased beneficial bacteria in colitic mice, and recruited CD11c+ dendritic cells (DCs) into inflamed colonic tissues. Colonic expression of regenerating islet-derived 3 gamma (Reg3g) was significantly increased by eckol. Overall, eckol represents a treatment alternative in colitis management. The induction of colonic Reg3g may account for the anti-inflammatory, anti-apoptotic, antimicrobial and immunoregulatory activities of eckol in colitic mice.