Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM—Increased serum CIIM in subjects with severe radiographic osteoarthritis
In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG 1053) by mass spectrometry. Two ELISAs against the neoepitope were develo...
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| Published in | Clinical biochemistry Vol. 44; no. 5; pp. 423 - 429 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier Inc
01.04.2011
Elsevier |
| Subjects | |
| Online Access | Get full text |
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| Abstract | In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.
A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG
1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (
n
=
51) and serum (
n
=
156). Knee OA was graded by standard Kellgren–Lawrence (KL) score.
The ELISAs showed good technical performance; CV%, <
13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (
P
<
0.05) in those individuals with mild or severe OA than in those with no OA.
We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen. |
|---|---|
| AbstractList | In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.
A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG
1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (
n
=
51) and serum (
n
=
156). Knee OA was graded by standard Kellgren–Lawrence (KL) score.
The ELISAs showed good technical performance; CV%, <
13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (
P
<
0.05) in those individuals with mild or severe OA than in those with no OA.
We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen. In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. The ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA. We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen. In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.OBJECTIVESIn joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score.METHODSA collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score.The ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA.RESULTSThe ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA.We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.CONCLUSIONWe developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen. |
| Author | Zheng, Qinlong Bay-Jensen, Anne-Christine Leeming, Diana J. Wang, Jianxia Qvist, Per Pedersen, Christian Karsdal, Morten A. Byrjalsen, Inger Li, Yi Dam, Erik B. Liu, Qi |
| Author_xml | – sequence: 1 givenname: Anne-Christine surname: Bay-Jensen fullname: Bay-Jensen, Anne-Christine email: acbj@nordicbioscience.com organization: Cartilage Biology and Biomarkers R&D, Nordic Bioscience, Herlev, Denmark – sequence: 2 givenname: Qi surname: Liu fullname: Liu, Qi organization: Assay Development, Nordic Bioscience China, Beijing, PR China – sequence: 3 givenname: Inger surname: Byrjalsen fullname: Byrjalsen, Inger organization: Cartilage Biology and Biomarkers R&D, Nordic Bioscience, Herlev, Denmark – sequence: 4 givenname: Yi surname: Li fullname: Li, Yi organization: Assay Development, Nordic Bioscience China, Beijing, PR China – sequence: 5 givenname: Jianxia surname: Wang fullname: Wang, Jianxia organization: Assay Development, Nordic Bioscience China, Beijing, PR China – sequence: 6 givenname: Christian surname: Pedersen fullname: Pedersen, Christian organization: Klinik Farsoe, Ortopaedkirugien, Farsoe, Denmark – sequence: 7 givenname: Diana J. surname: Leeming fullname: Leeming, Diana J. organization: Fibrosis Biology and Biomarker R&D, Nordic Bioscience, Herlev, Denmark – sequence: 8 givenname: Erik B. surname: Dam fullname: Dam, Erik B. organization: Synarc Imaging Technologies, Herlev, Denmark – sequence: 9 givenname: Qinlong surname: Zheng fullname: Zheng, Qinlong organization: Assay Development, Nordic Bioscience China, Beijing, PR China – sequence: 10 givenname: Per surname: Qvist fullname: Qvist, Per organization: Cartilage Biology and Biomarkers R&D, Nordic Bioscience, Herlev, Denmark – sequence: 11 givenname: Morten A. surname: Karsdal fullname: Karsdal, Morten A. organization: Cartilage Biology and Biomarkers R&D, Nordic Bioscience, Herlev, Denmark |
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| Keywords | MMP Cartilage OA Neoepitope Serum CIIM JSN Metalloproteinase Type II collagen Osteoarthritis ECM ELISA Diseases of the osteoarticular system Metalloendopeptidases Clinical biology Serum Degenerative disease Human Radiodiagnosis Enzyme Exploration Collagen type II Enzyme immunoassay Radiography Peptidases Articular cartilage Arthropathy Hydrolases Severe ELISA assay |
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A... In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential... |
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| SubjectTerms | Aged Aged, 80 and over Biological and medical sciences Cartilage Collagen Type II - blood Diseases of the osteoarticular system ELISA Enzyme-Linked Immunosorbent Assay - methods Female Humans Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Metalloproteases - blood Metalloproteinase Middle Aged Miscellaneous. Osteoarticular involvement in other diseases Neoepitope Osteoarthritis Osteoarthritis - blood Osteoarthritis - enzymology Serum CIIM Type II collagen |
| Title | Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM—Increased serum CIIM in subjects with severe radiographic osteoarthritis |
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