Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM—Increased serum CIIM in subjects with severe radiographic osteoarthritis

• Published in: Clinical biochemistry Vol. 44; no. 5; pp. 423 - 429
• Main Authors: Bay-Jensen, Anne-Christine, Liu, Qi, Byrjalsen, Inger, Li, Yi, Wang, Jianxia, Pedersen, Christian, Leeming, Diana J., Dam, Erik B., Zheng, Qinlong, Qvist, Per, Karsdal, Morten A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.04.2011; Elsevier
• Subjects: Aged; Aged, 80 and over; Biological and medical sciences; Cartilage; Collagen Type II - blood; Diseases of the osteoarticular system; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Female; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Metalloproteases - blood; Metalloproteinase; Middle Aged; Miscellaneous. Osteoarticular involvement in other diseases; Neoepitope; Osteoarthritis; Osteoarthritis - blood; Osteoarthritis - enzymology; Serum CIIM; Type II collagen; MMP; Cartilage; OA; Neoepitope; Serum CIIM; JSN; Metalloproteinase; Type II collagen; Osteoarthritis; ECM; ELISA; Diseases of the osteoarticular system; Metalloendopeptidases; Clinical biology; Serum; Degenerative disease; Human; Radiodiagnosis; Enzyme; Exploration; Collagen type II; Enzyme immunoassay; Radiography; Peptidases; Articular cartilage; Arthropathy; Hydrolases; Severe; ELISA assay
• ISSN: 0009-9120; 1873-2933; 1873-2933
• DOI: 10.1016/j.clinbiochem.2011.01.001

In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG 1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid ( n = 51) and serum ( n = 156). Knee OA was graded by standard Kellgren–Lawrence (KL) score. The ELISAs showed good technical performance; CV%, < 13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher ( P < 0.05) in those individuals with mild or severe OA than in those with no OA. We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.