Visualization of HDAC9 Spatiotemporal Subcellular Localization in Primary Neuron Cultures

Histone deacetylase (HDAC) 9 is one of class IIa HDACs which are expressed in developing cortical neurons. The translocation of HDAC9 from the nucleus to the cytoplasm is induced by neuronal activity during postnatal development, and is involved in regulation of various gene expressions. Visualizati...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1436; p. 119
Main Authors Sugo, Noriyuki, Yamamoto, Nobuhiko
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Animals
Cell Nucleus - metabolism
Cells, Cultured
Cloning, Molecular
Cytoplasm - metabolism
Gene Expression Regulation
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Mice
Microscopy, Fluorescence
Neurons - cytology
Neurons - metabolism
Neurons - ultrastructure
Protein Transport
Repressor Proteins - genetics
Repressor Proteins - metabolism
Time-Lapse Imaging - methods
Primary cortical neuron cultures
cDNA cloning
Transfection
Time-lapse imaging
HDAC9
Fluorescent imaging
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Summary:Histone deacetylase (HDAC) 9 is one of class IIa HDACs which are expressed in developing cortical neurons. The translocation of HDAC9 from the nucleus to the cytoplasm is induced by neuronal activity during postnatal development, and is involved in regulation of various gene expressions. Visualization of HDAC9 subcellular localization is a powerful tool for studying activity-dependent gene expression. Here, we describe a time-lapse imaging method using fluorescent protein-tagged HDAC9 in dissociated cortical neurons. This method reveals dynamic HDAC9-mediated gene expression in response to various signals.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3667-0_9