Distribution and Characterization of Immunoreactive Prolactin-Releasing Peptide (PrRP) in Rat Tissue and Plasma

• Published in: Biochemical and biophysical research communications Vol. 257; no. 2; pp. 264 - 268
• Main Authors: Matsumoto, Hirokazu, Murakami, Yuko, Horikoshi, Yasuko, Noguchi, Jiro, Habata, Yugo, Kitada, Chieko, Hinuma, Shuji, Onda, Haruo, Fujino, Masahiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.04.1999
• Subjects: Adrenal Glands - metabolism; Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Central Nervous System - metabolism; Chromatography, High Pressure Liquid; Female; Hypothalamic Hormones - blood; Hypothalamic Hormones - immunology; Hypothalamic Hormones - metabolism; Immunoenzyme Techniques; Male; Neuropeptides - blood; Neuropeptides - immunology; Neuropeptides - metabolism; Peptide Fragments - blood; Peptide Fragments - immunology; Peptide Fragments - metabolism; Pituitary Gland - metabolism; Prolactin-Releasing Hormone; Protein Isoforms - blood; Protein Isoforms - immunology; Protein Isoforms - metabolism; Rats; Rats, Wistar; Sensitivity and Specificity
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1999.0463

We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in the central nervous system and pituitary gland. The highest concentration of ir-PrRP was found in the hypothalamus. In peripheral tissues, appreciable levels of ir-PrRP were found only in the adrenal gland. The mean plasma concentration of ir-PrRP was 0.13 ± 0.01 fmol/ml (mean ± SEM). In reverse-phase and gel-filtration high performance liquid chromatography, hypothalamic ir-PrRP eluted at a position identical to that of PrRP31 and PrRP20. On the other hand, ir-PrRP from the adrenal gland and plasma eluted only at the position of synthetic PrRP31, indicating that molecular forms of ir-PrRPin vivodiffered among tissues.