Iron Promotes the Survival and Neurite Extension of Serum-starved PC12 Cells in the Presence of NGF by Enhancing Cell Attachment

• Published in: Molecules and cells Vol. 15; no. 1; pp. 10 - 19
• Main Authors: Hong, J.H, Noh, K.M, Yoo, Y.E, Choi, S.Y, Park, S.Y, Kim, Y.H, Chung, J. M. (Ewha Womans University, Seoul, Republic of Korea)
• Format: Journal Article
• Language: English
• Published: United States 한국분자세포생물학회 01.02.2003
• Subjects: Animals; Apoptosis - drug effects; Cell Adhesion - drug effects; Cell Survival - drug effects; Chromatin - drug effects; Chromatin - ultrastructure; Culture Media, Serum-Free; DNA Fragmentation; Ferrous Compounds - pharmacology; Integrin beta1 - physiology; IRON; Nerve Growth Factor - pharmacology; Neurites - drug effects; PC12 Cells - drug effects; PC12 Cells - ultrastructure; Rats; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1016/S1016-8478(23)13701-0

Delayed death of serum-starved PC12 cells on a poly-L-lysine (PLL) matrix was observed, even in the presence of NGE. NGF blocked the apoptotic death of attached but not detached cells, which suggests that delayed death may be related to cell detachment from the PLL matrix. Iron selectively blocked this anoikis like death by increasing cell attachment. Interestingly, the addition ofú+10 Ñ8M FeCl2 to the culture medium generated gelatinous iron precipitates, and the removal of the precipitates abolished the iron effect.