Echinochrome pigment extracted from sea urchin suppress the bacterial activity, inflammation, nociception, and oxidative stress resulted in the inhibition of renal injury in septic rats

• Published in: Journal of food biochemistry Vol. 46; no. 3; pp. e13729 - n/a
• Main Authors: Sadek, Shimaa A., Hassanein, Sarah S., Mohamed, Ayman S., Soliman, Amel M., Fahmy, Sohair R.
• Format: Journal Article
• Language: English
• Published: United States 01.03.2022
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Anti-Inflammatory Agents - pharmacology; cecal ligation and puncture; echinochrome; inflammation; Inflammation - drug therapy; Kidney; Nociception; Oxidative Stress; Rats; Sea Urchins; sepsis; Sepsis - complications; Sepsis - drug therapy; Sepsis - pathology; echinochrome; inflammation; oxidative stress; sepsis; cecal ligation and puncture
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/jfbc.13729

The current study aimed to evaluate the antibacterial, anti‐inflammatory, analgesic, and renoprotective effects of echinochrome pigment extracted from sea urchin. The disk diffusion method was used for the antibacterial activity of echinochrome against four different bacterial strains; Salmonella typhimurium, Pseudomonas aeroginosa, Staphylococcus aureus, and Listeria monocytogenes. While, acetic acid‐induced writhing, formalin‐induced licking, and hot plate latency assays evaluate the analgesic activity. The biochemical and oxidative stress markers of kidneys, as well as the histopathological examination, were measured to evaluate the renoprotective activity of echinochrome for cecal ligation and puncture‐induced renal injury in rats. Echinochrome pigment exhibited in vitro antibacterial activity against all aforementioned bacterial species besides a powerful anti‐inflammatory impact in vitro by the effective stabilization of the RBCs membrane and in vivo by decrease levels of serum IL6 and TNF‐α. What’s more, echinochrome showed a notable analgesic efficacy as well as an enhancement of the kidney’s biochemical markers, oxidative stress status, and histopathological screening. Ech attenuated cecal ligation and puncture‐induced renal injury by improving renal biomarkers, suppressing reactive oxygen species propagation as well as its antibacterial, anti‐inflammatory, and anti‐nociceptive activities. Practical applications Sea urchins are rich in pharmacologically important quinone pigments, specifically echinochrome. The current study aimed to evaluate the role of echinochrome as a renal protective remedy in sepsis and clarify its biological activities. Echinochrome exhibited antibacterial activity in vitro against Salmonella typhimurium, Pseudomonas aeroginosa, Staphylococcus aureus, and Listeria monocytogenes. Our results revealed that echinochrome protects the kidney against damage caused by sepsis in rats. Echinochrome can use in the treatment of sepsis as an antibacterial, anti‐inflammatory, and antioxidant agent. Echinochrome pigment treatment has a potential role in alleviating the sepsis. Antibacterial, anti‐inflammatory, and antioxidant activities, as well as analgesic potency of Echinochrome, could be regarded as potential mechanisms underlying the renoprotective efficacy of Echinochrome.