Angiotensin I-converting enzyme inhibitory activity of Acetes chinensis peptic hydrolysate and its antihypertensive effect in spontaneously hypertensive rats

• Published in: International journal of food science & technology Vol. 44; no. 10; pp. 2042 - 2048
• Main Authors: Zhang, Chaohua, Cao, Wenhong, Hong, Pengzhi, Ji, Hongwu, Qin, Xiaoming, He, Jinfeng
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2009; Blackwell Publishing Ltd; Wiley-Blackwell
• Subjects: Acetes chinensis; angiotensin I-converting enzyme inhibitory activity; antihypertensive effect; Biological and medical sciences; enzymatic hydrolysis; Food industries; Fundamental and applied biological sciences. Psychology; peptide absorption; ultrafiltration; angiotensin I-converting enzyme inhibitory activity; Peptides; Rat; Enzyme; Rodentia; enzymatic hydrolysis; peptide absorption; Hydrolysis; Vertebrata; Acetes chinensis; Mammalia; Absorption; Ultrafiltration; Hydrolysate; Angiotensin; antihypertensive effect; Enzymatic reaction
• ISSN: 0950-5423; 1365-2621
• DOI: 10.1111/j.1365-2621.2009.02028.x

Peptic hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from Acetes chinensis. The 3 kDa ultrafiltration filtrates (UF-IV) of the desalted hydrolysate exerted a potent ACE inhibitory activity with IC50 being 0.67 mg mL⁻¹. The fractions with molecular weight located between 611 and 240 Da achieved a total percentage of 63.6% in the UF-IV, with a mean peptide length of about 5.6-2.1. Gastrointestinal digestions were simulated on the hydrolysate and UF-IV and addressed their effect on the ACE inhibitory activity. The ACE inhibitory activity of the UF-IV could survive or be maintained in active form by simulated gastrointestinal digestion. Furthermore, the UF-IV exerted potent antihypertensive effect after oral administrations were given to spontaneously hypertensive rats (SHR) at a dose of 900 mg kg⁻¹ body weight. In conclusion, the UF-IV could serve as useful antihypertensive peptides in the prevention and treatment of hypertension and other associated disorders.