Singlet Oxygen Induces Fluorescent Proteins Dimerization

Lights out at two: The oxidation of avGFP and EGFP by singlet oxygen not only diminishes their fluorescence emission and changes their absorption properties, but also generates undesired protein aggregation. This could have important consequences in studies in which pairs of fluorescent proteins are...

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Published inChembiochem : a European journal of chemical biology Vol. 11; no. 17; pp. 2384 - 2388
Main Authors Valencia‐Perez, Adriana Z, Heyne, Belinda
Format Journal Article
LanguageEnglish
Published Weinheim Wiley‐VCH Verlag 22.11.2010
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
aggregation
Animals
Blotting, Western
green fluorescent protein
Green Fluorescent Proteins - chemistry
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hydrozoa - metabolism
Methylene Blue - chemistry
Methylene Blue - metabolism
Models, Molecular
Oxidation-Reduction
photochemistry
Protein Binding
Protein Multimerization
Protein Structure, Quaternary
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
singlet oxygen
Singlet Oxygen - chemistry
Singlet Oxygen - metabolism
Spectrometry, Fluorescence
Western blots
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Summary:Lights out at two: The oxidation of avGFP and EGFP by singlet oxygen not only diminishes their fluorescence emission and changes their absorption properties, but also generates undesired protein aggregation. This could have important consequences in studies in which pairs of fluorescent proteins are used, such as in FRET experiments.
Bibliography:http://dx.doi.org/10.1002/cbic.201000505
Natural Sciences and Engineering Research Council of Canada
ArticleID:CBIC201000505
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ark:/67375/WNG-D2KCSF8Q-M
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201000505