Singlet Oxygen Induces Fluorescent Proteins Dimerization
Lights out at two: The oxidation of avGFP and EGFP by singlet oxygen not only diminishes their fluorescence emission and changes their absorption properties, but also generates undesired protein aggregation. This could have important consequences in studies in which pairs of fluorescent proteins are...
Saved in:
Published in | Chembiochem : a European journal of chemical biology Vol. 11; no. 17; pp. 2384 - 2388 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley‐VCH Verlag
22.11.2010
WILEY-VCH Verlag WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Lights out at two: The oxidation of avGFP and EGFP by singlet oxygen not only diminishes their fluorescence emission and changes their absorption properties, but also generates undesired protein aggregation. This could have important consequences in studies in which pairs of fluorescent proteins are used, such as in FRET experiments. |
---|---|
Bibliography: | http://dx.doi.org/10.1002/cbic.201000505 Natural Sciences and Engineering Research Council of Canada ArticleID:CBIC201000505 istex:057F534F24B803709D6359F4ADB72C45F044054A ark:/67375/WNG-D2KCSF8Q-M ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1439-4227 1439-7633 |
DOI: | 10.1002/cbic.201000505 |