Evidence that the Ipl1-Sli15 (Aurora Kinase-INCENP) Complex Promotes Chromosome Bi-orientation by Altering Kinetochore-Spindle Pole Connections

How sister kinetochores attach to microtubules from opposite spindle poles during mitosis (bi-orientation) remains poorly understood. In yeast, the ortholog of the Aurora B-INCENP protein kinase complex (Ipl1-Sli15) may have a role in this crucial process, because it is necessary to prevent attachme...

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Published inCell Vol. 108; no. 3; pp. 317 - 329
Main Authors Tanaka, Tomoyuki U., Rachidi, Najma, Janke, Carsten, Pereira, Gislene, Galova, Marta, Schiebel, Elmar, Stark, Michael J.R., Nasmyth, Kim
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 08.02.2002
Subjects
Aurora Kinases
Chromosomal Proteins, Non-Histone - physiology
Chromosomes, Fungal - physiology
Chromosomes, Fungal - ultrastructure
DNA Replication
DNA, Fungal - physiology
Intracellular Signaling Peptides and Proteins
Ipl1 protein
kinetochores
Kinetochores - physiology
Kinetochores - ultrastructure
Mitosis - physiology
Mutation
Protein Kinases - physiology
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins
Sli15 protein
Spindle Apparatus - physiology
Spindle Apparatus - ultrastructure
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Summary:How sister kinetochores attach to microtubules from opposite spindle poles during mitosis (bi-orientation) remains poorly understood. In yeast, the ortholog of the Aurora B-INCENP protein kinase complex (Ipl1-Sli15) may have a role in this crucial process, because it is necessary to prevent attachment of sister kinetochores to microtubules from the same spindle pole. We investigated IPL1 function in cells that cannot replicate their chromosomes but nevertheless duplicate their spindle pole bodies (SPBs). Kinetochores detach from old SPBs and reattach to old and new SPBs with equal frequency in IPL1+ cells, but remain attached to old SPBs in ipl1 mutants. This raises the possibility that Ipl1-Sli15 facilitates bi-orientation by promoting turnover of kinetochore-SPB connections until traction of sister kinetochores toward opposite spindle poles creates tension in the surrounding chromatin.
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ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(02)00633-5