Correlation analysis between vaginal microecology and high-risk human papillomavirus (HR-HPV)-positive cervical squamous intraepithelial lesions (SIL)

• Published in: Medicine (Baltimore) Vol. 104; no. 27; p. e42914
• Main Authors: Zeng, Baojin, Ren, Xiuling, Cheng, Yali, Wang, Chunhua, Li, Jiawei, Jiang, Li, Zhang, Shuai, Chen, Sisi, Yu, Danjun, Lin, Jingjing
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 04.07.2025
• Subjects: Adult; Female; Human Papillomavirus Viruses; Humans; Middle Aged; Observational Study; Papillomaviridae - isolation & purification; Papillomavirus Infections - complications; Papillomavirus Infections - epidemiology; Papillomavirus Infections - virology; Risk Factors; Squamous Intraepithelial Lesions - microbiology; Squamous Intraepithelial Lesions - pathology; Squamous Intraepithelial Lesions - virology; Squamous Intraepithelial Lesions of the Cervix - microbiology; Squamous Intraepithelial Lesions of the Cervix - pathology; Squamous Intraepithelial Lesions of the Cervix - virology; Uterine Cervical Dysplasia - virology; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; Vagina - microbiology; Vagina - virology; vaginal microecology; high-risk human papillomavirus; low-grade squamous intraepithelial lesions; high-grade squamous intraepithelial lesions
• ISSN: 1536-5964; 0025-7974; 1536-5964
• DOI: 10.1097/MD.0000000000042914

This study is aimed to investigate the correlation between vaginal microecology and high-risk human papillomavirus (HR-HPV)-positive cervical squamous intraepithelial lesions (SIL) using the regression analysis. Patients (n = 372) with HR-HPV-positive from January 2020 to June 2022 were recruited after preliminary confirmation by colposcopy, HPV test, and typing, as well as loop electrosurgical excision procedure. Based on the pathological results, the recruited subjects were divided into 3 groups, that is, negative for intraepithelial lesion or malignancy, low-grade SIL, and high-grade SIL (HSIL). Finally, the clinical factors, virological data, and vaginal microecological changes of the 3 experimental groups were analyzed. Age was identified as a significant risk factor for HSIL, with an OR of 1.048 (95% CI: 1.006-1.094 and P = .026). Various HR-HPV types (HPV16, HPV18, and HPV52) were closely associated with HSIL, with multiple infections significantly increasing the risk (odds ratio, OR: 5.810, P = .04). The changes in the vaginal microecology were strongly associated with HSIL, including elevated pH (>4.5), reduced hydrogen peroxide levels, and increased bacterial vaginosis (BV) prevalence. BV demonstrated a sensitivity of 66.10% and a specificity of 70.31% for predicting HSIL. Furthermore, decreased Lactobacillus levels (OR: 3.20, P < .001) showed their protective role, while elevated sialidase activity (OR: 5.610, P = .002) emerged as a significant risk factor. Accordingly, the key independent predictors for low-grade SIL and HSIL included age, infection type, pH, microbiome density, BV, and sialidase activity. The mixed infection of HPV16, HPV18, HPV52, and HPV resulting in cervical SILs could be closely related to the vaginal microecology.