Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT

• Published in: Pathogens and disease Vol. 68; no. 1; pp. 12 - 19
• Main Authors: Hirai, Kimito, Maeda, Hiroshi, Omori, Kazuhiro, Yamamoto, Tadashi, Kokeguchi, Susumu, Takashiba, Shogo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2013; Oxford University Press
• Subjects: Adult; Aged; Antibodies; Antibodies, Bacterial - blood; Antibody response; Archaea; Autoantibodies; Autoantibodies - blood; autoantibody; autoimmune disease; Autoimmune diseases; Autoimmune Diseases - blood; Autoimmune Diseases - immunology; Bacterial Proteins - immunology; chaperonin; Chaperonin 60 - immunology; Chaperonin 60 - pharmacology; Chaperonin Containing TCP-1 - immunology; Chaperonins; Cross-reactivity; Disease control; Female; Gum disease; Hsp60 protein; Humans; Immune system; Immunoglobulin G - blood; Male; Methanobrevibacter - immunology; Methanobrevibacter oralis; Middle Aged; Pathogenesis; Periodontitis; Periodontitis - blood; Periodontitis - immunology; Protein Subunits - immunology; Recombinant Proteins - immunology; Recombinant Proteins - pharmacology; Western blotting; autoantibody; autoimmune disease; chaperonin; periodontitis
• ISSN: 2049-632X; 2049-632X
• DOI: 10.1111/2049-632X.12041

Abstract Both group I (HSP60) and group II (CCT) chaperonins are targets of autoantibodies. Autoimmune reactions to HSP60 have been well characterized, while immune reactions to group II chaperonin have not been clarified. Methanobrevibacter oralis is a suspected periodontal pathogen with group II chaperonin. In this study, serum responses to M. oralis chaperonin, human HSP60, and CCT subunits were examined using sera from patients with periodontitis and autoimmune diseases. In comparison with healthy controls, periodontitis patients showed significantly higher responses to CCT4 and CCT8 on dot blot analysis. Signals for CCT3 and CCT8 in autoimmune disease patients were significantly higher than in controls. Significant differences were also demonstrated by Western blotting in anti-CCT4 response in both patient groups. All subjects showed strong reactivity to M. oralis chaperonin and faint signals to human HSP60. Autoantibodies were raised against CCT rather than HSP60; and CCT3, CCT4, and CCT8 were shown to be the main targets. Host immune systems may be frequently exposed to chaperonins of Archaea in various habitats. Although further studies of the cross-reactivity between M. oralis chaperonin and human CCT are required, anti-CCT autoantibodies may be involved in the pathogenesis of periodontitis and autoimmune diseases. Methanobrevibacter oralis is a suspected periodontal pathogen and in this study the serum responses to human CCT and the M.oralis group II chaperonin were studied in patients with periodontitis and autoimmune diseases. Interestingly, both diseased groups showed elevated responses to one or more CCT subunits, suggesting the potential importance of cross-reactivity in autoimmune diseases.