Histochemical analysis of changes in lectin binding in murine glomerular lesions

Lectin binding in diseased murine glomeruli was studied in MRL/1 mice, using seven different fluorescence- or peroxidase-coupled lectins: Griffonia simplicifolia I (GS-I), Ulex europaeus agglutinin I (UEA-I), Ricinus communis agglutinin I (RCA-I), wheat germ agglutinin (WGA), concanavalin A (Con A),...

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Bibliographic Details
Published inActa pathologica japonica Vol. 39; no. 1; p. 31
Main Authors Kizaki, T, Takeda, Z, Watanabe, M, Hanioka, K, Itoh, H
Format Journal Article
LanguageEnglish
Published Australia 01.01.1989
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Summary:Lectin binding in diseased murine glomeruli was studied in MRL/1 mice, using seven different fluorescence- or peroxidase-coupled lectins: Griffonia simplicifolia I (GS-I), Ulex europaeus agglutinin I (UEA-I), Ricinus communis agglutinin I (RCA-I), wheat germ agglutinin (WGA), concanavalin A (Con A), peanut agglutinin (PNA), and Helix pomatia agglutinin (HPA). Lectin binding in diseased glomeruli of MRL/1 mice was different from that in normal glomeruli. Light and fluorescence microscopy showed that: 1. in mesangial proliferative lesions, the binding of RCA-I, WGA and Con A increased and that of GS-I and PNA appeared in the mesangium; 2. in other glomerular lesions, UEA-I binding appeared and RCA-I stained the altered membranes irregularly. Electron microscopy showed that: 1. GS-I stained the endothelial cell coat and the glomerular basement membrane covered by the endothelial cells; 2. GS-I strongly stained the dilated subendothelium in regions of mild mesangial interposition; 3. GS-I stained the cell coat of invasive macrophages; 4. GS-I and UEA-I stained the cell membrane-like material derived from degenerative endothelial cells; 5. RCA-I stained the epithelial and endothelial cell coats and the glomerular basement membrane. These results indicate that lectin-binding studies can be used for analysis of glomerular lesions.
ISSN:0001-6632
DOI:10.1111/j.1440-1827.1989.tb02400.x