Residence time of netilmicin in tears

To determine the concentration of netilmicin in tears after eye drop administration. A clinical study was carried out on 32 healthy volunteers. Subjects were divided into four groups and underwent tear collection at 5, 10, 20, and 60-minutes after drug administration, respectively. Tear samples were...

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Bibliographic Details
Published inCornea Vol. 21; no. 1; p. 48
Main Authors Scuderi, Anna Claudia, De Lazzari, Alessandra, Miano, Fausto, Zola, Pietro
Format Journal Article
LanguageEnglish
Published United States 01.01.2002
Subjects
Adolescent
Adult
Aged
Anti-Bacterial Agents - pharmacokinetics
Biological Availability
Child
Chromatography, High Pressure Liquid
Female
Half-Life
Humans
Male
Middle Aged
Netilmicin - pharmacokinetics
Tears - metabolism
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Summary:To determine the concentration of netilmicin in tears after eye drop administration. A clinical study was carried out on 32 healthy volunteers. Subjects were divided into four groups and underwent tear collection at 5, 10, 20, and 60-minutes after drug administration, respectively. Tear samples were collected through capillary suction from the inferior conjunctival "cul-de-sac" and analyzed by high-pressure liquid chromatography (HPLC). Netilmicin concentration in tears decreased after a first order kinetics, a best-fit curve was drawn and the minimum inhibitory concentration (MIC90) intersection for the most common ocular pathogens was calculated. The extrapolation of the curve shows that the concentration of netilmicin on the ocular surface can be effective against microorganisms more than 120 minutes after eye drop instillation.
ISSN:0277-3740
DOI:10.1097/00003226-200201000-00011