Is red blood cell rheology preserved during routine blood bank storage?
BACKGROUND: Red blood cell (RBC) units stored for more than 2 weeks at 4°C are currently considered of impaired quality. This opinion has primarily been based on altered RBC rheologic properties (i.e., enhanced aggregability, reduced deformability, and elevated endothelial cell interaction), during...
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Published in | Transfusion (Philadelphia, Pa.) Vol. 50; no. 4; pp. 941 - 948 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Malden, USA
Blackwell Publishing Inc
01.04.2010
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Subjects | |
Online Access | Get full text |
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Summary: | BACKGROUND: Red blood cell (RBC) units stored for more than 2 weeks at 4°C are currently considered of impaired quality. This opinion has primarily been based on altered RBC rheologic properties (i.e., enhanced aggregability, reduced deformability, and elevated endothelial cell interaction), during prolonged storage of nonleukoreduced RBC units. In this study, the rheologic properties and cell variables of leukoreduced RBC units, during routine blood bank storage in saline‐adenine‐glucose‐mannitol, were investigated.
STUDY DESIGN AND METHODS: Ten leukoreduced RBC units were stored at the blood bank for 7 weeks at 4°C. RBCs were tested weekly for aggregability, deformability, and other relevant variables.
RESULTS: RBC aggregability was significantly reduced after the first week of storage but recovered during the following weeks. After 7 weeks aggregability was slightly, but significantly, reduced (46.9 ± 2.4‐44.3 ± 2.2 aggregation index). During storage the osmotic fragility was not significantly enhanced (0.47 ± 0.01% phosphate‐buffered saline) and the deformability at shear stress of 3.9 Pa was not significantly reduced (0.36 ± 0.01 elongation index [EI]). The deformability at 50 Pa was reduced (0.58 ± 0.01‐0.54 ± 0.01 EI) but remained within reference values (0.53 ± 0.04). During 5 weeks of storage, adenosine triphosphate was reduced by 54% whereas mean cell volume, pH, and mean cell hemoglobin concentration were minimally affected.
CONCLUSIONS: RBC biochemical and physical alterations during storage minimally affected the RBC ability to aggregate and deform, even after prolonged storage. The rheologic properties of leukoreduced RBC units were well preserved during 7 weeks of routine blood bank storage. |
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Bibliography: | ArticleID:TRF2521 istex:F1F9A97FEED497737E78A276009718942935118F ark:/67375/WNG-23LLS477-F Source of support: government. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0041-1132 1537-2995 |
DOI: | 10.1111/j.1537-2995.2009.02521.x |