Modeling the electrophoresis and transport of peptides: The effective sphere model and complex formation

• Published in: Journal of separation science Vol. 33; no. 16; pp. 2439 - 2446
• Main Authors: Allison, Stuart A, Pei, Hongxia, Allen, Michelle, Brown, Jocelyn, Kim, Chang-Il, Zhen, Yang
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.08.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Biological Transport; Electrophoresis, Capillary; Electrophoretic mobility of peptides; Hydrogen-Ion Concentration; Modeling peptide transport; Models, Chemical; Peptide mobility; Peptides - isolation & purification
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201000130

Modeling the electrophoretic mobility of peptides is examined in this study using a "coarse grained" bead model, or B model for short 8 and also a simpler "effective sphere" (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013 M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.