The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment
Seifrtová M, Havelek R, Ćmielová J, Jiroutová A, Soukup T, Brůčková L, Mokrý J, English D, Řezáčová M. The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment. International Endodontic Journal, 45, 401–412, 2012. Aim To determine the response of dental pulp stem cells (D...
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Published in | International endodontic journal Vol. 45; no. 5; pp. 401 - 412 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.05.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Seifrtová M, Havelek R, Ćmielová J, Jiroutová A, Soukup T, Brůčková L, Mokrý J, English D, Řezáčová M. The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment. International Endodontic Journal, 45, 401–412, 2012.
Aim To determine the response of dental pulp stem cells (DPSCs) to DNA‐damaging cytostatic cisplatin and compare it with the response of normal human dermal fibroblasts (HDFs).
Methodology Dental pulp stem cells were exposed to 5, 10, 20 or 40 μmol L−1 of cisplatin. The proliferation of affected cells was assessed by a Z2 Counter and viability was assessed by means of a Vi‐Cell XR using Trypan blue exclusion staining. Cell cycle analysis and induction of apoptosis were performed by flow cytometry. Induction of apoptosis was determined by monitoring the activities of caspases. The expression of proteins was detected by electrophoresis and Western blotting. The descriptive statistics of the results was analyzed by Student’s t‐test.
Results Dental pulp stem cells had a greater genotoxic stress response to cisplatin compared to HDFs. All three main Mitogen‐activated protein kinases (MAPK) families – extracellular signal‐regulated kinases (ERK), c‐Jun‐N‐terminal kinase (JNK) and p38 were activated after treatment of DPSCs with cisplatin. The activation of MAPK pathways was not observed in HDFs exposed to cisplatin. The exposure of DPSCs and HDFs to cisplatin provoked an increase in p53 and p21 expression and p53 phosphorylation of serine 15. Higher concentrations of cisplatin reduced the viability of DPSCs and HDFs and induced the activation of caspases 3/7 and 9.
Conclusion Dental pulp stem cells had a greater genotoxic stress response to cisplatin compared to HDFs. Cisplatin in higher concentrations triggered activation of MAPK and apoptosis in DPSCs but not in HDFs. |
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Bibliography: | istex:3F106DC08E1D47BF50AE92F520D757748AF9E861 ark:/67375/WNG-RVZ1ZDMF-Q ArticleID:IEJ1990 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0143-2885 1365-2591 |
DOI: | 10.1111/j.1365-2591.2011.01990.x |