Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

• Published in: Annals of surgical oncology Vol. 5; no. 8; pp. 699 - 705
• Main Authors: Rijken, A M, Umezawa, A, van Gulik, T M, Bosma, A, Polak, M M, Offerhaus, G J, Obertop, H, Gouma, D J
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.12.1998
• Subjects: 2-Methylisoborneol; Adult; Aged; Antigens; Bile Duct Neoplasms - pathology; Bile Duct Neoplasms - surgery; Bile ducts; Carcinoma; Cell growth; Cell proliferation; Cholangiocarcinoma - pathology; Cholangiocarcinoma - surgery; Deoxyribonucleic acid; Diploids; DNA; DNA, Neoplasm - analysis; Female; Flow Cytometry; Humans; Immunohistochemistry; Ki-67 Antigen - analysis; Male; Middle Aged; Ploidy; Prognosis; Survival Analysis; Tumors
• ISSN: 1068-9265; 1534-4681
• DOI: 10.1007/bf02303480

The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. A significant correlation was found between a low MIB-1 index (<20%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20% was associated with decreased survival time.