Inhibition of vaccinia virus replication by cyclosporin A analogues correlates with their affinity for cellular cyclophilins
CR Damaso and N Moussatche Laboratorio de Biologia Molecular de Virus, Instituto de Biofisica Carlos Chagas Filho, CCS, UFRJ, Rio de Janeiro, Brazil. The mechanism by which cyclosporin A (CsA) inhibits vaccinia virus (VV) replication is still unclear. The present study addresses the question of whet...
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Published in | Journal of general virology Vol. 79; no. 2; pp. 339 - 346 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Soc General Microbiol
01.02.1998
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Subjects | |
Online Access | Get full text |
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Summary: | CR Damaso and N Moussatche
Laboratorio de Biologia Molecular de Virus, Instituto de Biofisica Carlos Chagas Filho, CCS, UFRJ, Rio de Janeiro, Brazil.
The mechanism by which cyclosporin A (CsA) inhibits vaccinia virus (VV)
replication is still unclear. The present study addresses the question of
whether CsA-binding proteins named cyclophilins (Cyps) are involved in the
anti-VV activity of CsA. Six CsA analogues were analysed, and their
affinity for Cyps in VV-infected BSC-40 cells and their potency as
inhibitors of VV replication were evaluated. It was demonstrated that
analogues with strong Cyp-binding activity, such as CsC, CsG and
[MeAla6]CsA, also exhibit a strong antiviral effect. In contrast, drugs
with low ([MeBm2t1]CsA and CsH) or no ([MeLeu11]CsA) affinity for Cyps show
poor or no antiviral activity. The data obtained suggest a correlation
between the ability of CsA to block VV replication and Cyp binding
activity, and indicate the involvement of Cyps in the VV replicative cycle.
They also suggest that the anti-VV action of CsA may occur by a pathway
distinct from that involved in the immunosuppressive effect of the drug. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-79-2-339 |