Using 2', 7'-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents

• Published in: Molecular biotechnology Vol. 19; no. 2; pp. 125 - 131
• Main Authors: BLAND, E. J, KESHAVARZ, T, BUCKE, C
• Format: Journal Article
• Language: English
• Published: Totowa, NJ Humana Press 01.10.2001; Springer Nature B.V
• Subjects: 2',7'-dichlorodihydrofluorescein-diacetate; Adjuvants, Immunologic - pharmacology; Biological and medical sciences; Blood; Cells; Coloring Agents - pharmacology; Fluoresceins - pharmacology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Immunology; immunomodulating agents; Infection; Leukocytes (neutrophilic); Leukocytes (polymorphonuclear); Models, Chemical; Modulation of the immune response (stimulation, suppression); Neutrophils - metabolism; Polysaccharides; Polysaccharides - chemistry; Reactive Oxygen Species; Saccharides; Saccharomyces cerevisiae - metabolism; Salmonella typhimurium - metabolism; Spectrometry, Fluorescence; Studies; Time Factors; Human; Chronic; Immunomodulation; Leukocyte; Granulomatosis; Method; Neutrophil; Real time; Polysaccharide; Free radical; Quantitative analysis
• ISSN: 1073-6085; 1559-0305; 1073-6085
• DOI: 10.1385/MB:19:2:125

Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.