Conversion in the peptides coating cadmium:sulfide crystallites in Candida glabrata

• Published in: Journal of inorganic biochemistry Vol. 48; no. 2; pp. 95 - 105
• Main Authors: Barbas, J., Ellis, W.R., Santhanagopalan, V., Blaszczynski, M., Winge, D.R.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.11.1992; Elsevier Science
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; CADMIO; CADMIUM; Cadmium - chemistry; Cadmium Compounds; Candida - metabolism; Crystallization; DESINTOXICACION; DETOXIFICATION; Fundamental and applied biological sciences. Psychology; Glutathione - chemistry; Inorganic compounds; Kinetics; METABOLISME; METABOLISMO; Other biological molecules; PEPTIDE; Peptides - chemistry; PEPTIDOS; Sulfides; SULFURE; SULFUROS; TORULOPSIS; Fungi; Cadmium; Sulfides; Crystallites; Candida glabrata; Fungi Imperfecti; Thallophyta
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/0162-0134(92)80019-R

Cultures of Candida glabrata treated with CdCl 2 form intracellular Cd(II) complexes that evolve with the time of culturing. Initially, glutathione (γECG) appears to be the major buffering component. One type of Cd(II)-glutathione complex exists as a cadmium:sulfide (CdS) crystallite coated with glutathione. A time dependent change in the coating of the CdS particles occurs with a decrease in the (γECG) content and a corresponding increase in the abundance of (γEC)nG peptides with (γEC) 2G becoming the predominant peptide. The desGly variant (γEC) 2 appears in significant concentration only in late cultures. The evolution in isopeptide coating appears to be dependent on the sulfide content of the CdS particles. Cellular conditions that enhance the generation of sulfide ions facilitate the conversion from γECG to (γEC) 2G.