Antinociceptive properties of ethanolic extract and fractions of Pterodon pubescens Benth. seeds

• Published in: Journal of ethnopharmacology Vol. 98; no. 1; pp. 109 - 116
• Main Authors: Coelho, Luciana Pontes, Reis, Patrícia Alves, de Castro, Fernanda Lima, Gayer, Carlos Roberto Machado, Lopes, Carlos da Silva, Silva, Maria Cristina da Costa e, Sabino, Kátia Costa de Carvalho, Todeschini, Adriane Regina, Coelho, Marsen Garcia Pinto
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 08.04.2005; Elsevier
• Subjects: Abdomen; Abdominal constriction; Administration, Oral; analgesic effect; Analgesics - chemistry; Analgesics - isolation & purification; Analgesics - pharmacology; Animals; Antinociception; Aspirin - pharmacology; Biological and medical sciences; Chemical Fractionation - methods; Dipyrone - pharmacology; Diterpenes - chemistry; Diterpenes - isolation & purification; Diterpenes - pharmacology; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical - methods; Ethanol; Fabaceae; Formaldehyde - administration & dosage; Formaldehyde - adverse effects; Formaldehyde - antagonists & inhibitors; Formalin test; Gas Chromatography-Mass Spectrometry - methods; General pharmacology; Inflammation - chemically induced; Inflammation - prevention & control; Male; Mass spectrometry; Medical sciences; medicinal plants; Mice; Morphine - pharmacology; Pain Measurement - drug effects; Pain Measurement - methods; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Oils - administration & dosage; Plant Oils - chemistry; Plant Oils - pharmacology; Pterodon; Pterodon pubescens; seed extracts; seed oils; Seeds - chemistry; Sesquiterpenes - chemistry; Sesquiterpenes - isolation & purification; Sesquiterpenes - pharmacology; Tail - drug effects; Tail - injuries; Temperature; traditional medicine; Abdominal constriction; Antinociception; Pterodon pubescens; Mass spectrometry; Formalin test; Analgesic; Seeds; Pharmacognosy; Plant origin; Extract; Medicinal plant
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2005.01.014

We have previously demonstrated that the hydroalcoholic extract from Pterodon pubescens Benth. seeds (sucupira branca, Leguminosae) exhibits anti-arthritic activity and that its oleaginous extract (OEP) and PF1 fraction exhibit acute and topic anti-edematogenic activities. In this work, we studied the antinociceptive activity of OEP and its fractions on the acetic acid-induced abdominal constriction and formalin assays in SW male mice. OEP was obtained by ethanol extraction and its four fractions by sequential liquid–liquid extraction. PF2 GC/MS profile indicated it contains furane diterpenes derivatives of vouacapan and non-vouacapan compounds. The antinociceptive properties were demonstrated to OEP and predominantly to PF1 and PF2 by the writhing test. In the formalin assay, PF1 inhibited both phases and PF2 inhibited mainly the late one. Then, PF1 and PF2 seemed to present antinociceptive effects by different mechanisms, peripheral and/or central inhibitory ones, and showed maximum antinociceptive properties with very low doses, providing a rationale for its popular use in pain disorders.