Differences in TLR9-dependent inhibitory effects of H2O2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species

• Published in: Microbes and infection Vol. 15; no. 2; pp. 96 - 104
• Main Authors: Hiramatsu, Yukihiro, Satho, Tomomitsu, Irie, Keiichi, Shiimura, Shota, Okuno, Takahiro, Sharmin, Tanjina, Uyeda, Saori, Fukumitsu, Yuki, Nakashima, Yukihiko, Miake, Fumio, Kashige, Nobuhiro
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.02.2013
• Subjects: Anti-inflammatory effect; Genomic DNA; Lactic acid bacteria; Lactobacillus casei; NF-κB; Toll-like receptor 9; NF-κB; Anti-inflammatory effect; Lactic acid bacteria; Toll-like receptor 9; Genomic DNA
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/j.micinf.2012.11.003

Lactic acid bacteria (LAB) show anti-inflammatory effects, and their genomic DNA was identified as one of the anti-inflammatory components. Despite the differences in anti-inflammatory effects between live LAB dependent not only on genus but also species, this effect has not been compared at the genomic DNA level. We compared the anti-inflammatory effects of the genomic DNA from five Lactobacillus species—Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus plantarum, and Lactobacillus reuteri—using Caco-2 cells. To evaluate anti-inflammatory effects, decreases in H2O2-induced IL-8 secretion and inhibition of H2O2-induced NF-κB/IκB-α system activation were examined. All LAB genomic DNAs dose-dependently decreased H2O2-induced IL-8 secretion and inhibited H2O2-induced NF-κB/IκB-α system activation. Comparison of these effects between Lactobacillus species showed that the anti-inflammatory effects of L. acidophilus genomic DNA are lower than those of the other species. Furthermore, suppression of Toll-like receptor 9 (TLR9), a specific receptor of bacterial DNA, expression by RNAi abolished the decrease of H2O2-induced IL-8 secretion and inhibition of H2O2-induced NF-κB/IκB-α system activation by LAB genomic DNA. Our results demonstrated that the anti-inflammatory effects of genomic DNA differ between Lactobacillus species and TLR9 is one of the major pathways responsible for the anti-inflammatory effect of LAB genomic DNA.