Molecular Characterization of Seizure-Related Genes Isolated by Differential Screening

To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confir...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 219; no. 3; pp. 795 - 799
Main Authors Kajiwara, Kagemasa, Nagawawa, Hideko, Shimizu-Nishikawa, Keiko, Ookura, Tamiko, Kimura, Minoru, Sugaya, Eiichi
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 27.02.1996
Subjects
Amino Acid Sequence
Animals
Calcium - metabolism
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Cells, Cultured
Cloning, Molecular
DNA, Complementary
Female
Gene Expression
Genes, fos
Heat-Shock Proteins
Male
Mice
Mice, Inbred C57BL
Molecular Chaperones - biosynthesis
Molecular Chaperones - genetics
Molecular Sequence Data
Neurons - drug effects
Neurons - physiology
Oocytes - physiology
Pentylenetetrazole - pharmacology
Phospholipases A - biosynthesis
Phospholipases A - genetics
Phospholipases A2
Proto-Oncogene Proteins c-fos - biosynthesis
Recombinant Proteins - biosynthesis
Seizures - genetics
Seizures - physiopathology
Sequence Homology
Xenopus
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Summary:To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of differential screening procedure: 6 clones showed increased mRNA level and 3 clones showed decreased expression with PTZ. Interestingly, 4 genes which were isolated by this technique were related to intracellular calcium action. They were cytosolic phospholipase A2, 78 kDa glucose regulated protein, SEZ-15 which has an EF hand motif and PTZ-17 that causes calcium current inXenopusoocyte with PTZ application. These data and our previous results suggest that intracellular calcium may play an important role for seizure-related pathophysiological changes in neuronal cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0313