Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tilia cordata Mill.)

• Published in: Plant cell reports Vol. 9; no. 7; pp. 398 - 401
• Main Author: Chalupa, V
• Format: Journal Article
• Language: English
• Published: Germany 01.11.1990
• Subjects: culture media; embryo (plant); embryo culture; plant growth substances; Quercus petraea; Quercus robur; regenerative ability; somatic embryogenesis; Tilia cordata
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/bf00232408

Embryogenic cultures and somatic embryos were obtained from immature zygotic embryos of oak (Quercus robur L.) cultured on a modified MS medium and WPM containing BAP (1 mg·l(-1)) and GA3 (1 mg·l(-1)) or BAP and IBA. Germination and conversion of oak somatic embryos into plantlets was achieved on WPM containing a reduced concentration of cytokinin. Linden (Tilia cordata Mill.) somatic embryos developed in embryogenic tissues initiated from immature zygotic embryos cultured on a modified MS medium supplemented with 2,4-D (0.3-2.0 mg·l(-1)). Germination of linden somatic embryos and plantlet formation occurred on MS medium containing a low concentration of IBA. Oak and linden plantlets produced from somatic embryos were successfully established in soil. Somatic embryos and plantlets were also regenerated from embryogenic cultures of Quercus petraea and Tilia platyphyllos.