Enantioselective Effects of Methamidophos on the Coelomocytes Lysosomal Membrane Stability of Eisenia fetida

• Published in: Bulletin of environmental contamination and toxicology Vol. 89; no. 6; pp. 1161 - 1164
• Main Authors: Chen, Linhua, Lu, Xianting, Ma, Yun
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.12.2012; Springer Nature B.V
• Subjects: Animals; Aquatic Pollution; Earth and Environmental Science; Ecotoxicology; Eisenia fetida; Environment; Environmental Chemistry; Environmental Health; Environmental testing; Indicator organisms; Insecticides; Insecticides - chemistry; Insecticides - toxicity; Intracellular Membranes - drug effects; Liquid chromatography; Lysosomes - drug effects; Membranes; Oligochaeta; Organophosphates; Organothiophosphorus Compounds - chemistry; Organothiophosphorus Compounds - toxicity; Pollution; Retention; Soil Pollutants - chemistry; Soil Pollutants - toxicity; Soil Science & Conservation; Stereoisomerism; Waste Water Technology; Water Management; Water Pollution Control; Worms; Earthworm; Neutral red; Chiral; Methamidophos
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-012-0861-y

Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (−)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.