Social stress-induced hypothyroidism is attenuated by antidepressant treatment in rats

• Published in: Neuropharmacology Vol. 62; no. 1; pp. 446 - 456
• Main Authors: Olivares, Emerson L., Silva-Almeida, Cláudio, Pestana, Fernanda M., Sonoda-Côrtes, Rafael, Araujo, Iracema G., Rodrigues, Nayana C., Mecawi, André S., Côrtes, Wellington S., Marassi, Michelle P., Reis, Luis Carlos, Rocha, Fábio F.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2012
• Subjects: Adipose Tissue, Brown - drug effects; Adipose Tissue, Brown - pathology; Analysis of Variance; Animals; Antidepressive Agents - therapeutic use; Body Weight - drug effects; Corticosterone; Corticosterone - blood; Deiodinases; Depression; Disease Models, Animal; Exploratory Behavior - drug effects; Fluoxetine - therapeutic use; Food Preferences - drug effects; Hypothyroidism - drug therapy; Hypothyroidism - etiology; Hypothyroidism - pathology; Male; Organ Size - drug effects; Radioimmunoassay; Rats; Rats, Wistar; Social Behavior; Stress; Stress, Psychological - complications; Stress, Psychological - drug therapy; Sucrose - administration & dosage; Thyroid hormone; Thyroxine - blood; Time Factors; Triiodothyronine; Rats; Thyroid hormone; Depression; Corticosterone; Stress; Deiodinases
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2011.08.035

Although serotonergic system has been classically implicated in mood modulation, there has been relatively little study on the relationship between this system and thyroid hormones (TH) economy in stress models. When TH are studied, the effects of stress on thyroid function seems to be complex and depend on the kind and time of stress which counts for the elusiveness of mechanisms underlying changes in TH economy. Herein, we hypothesized that serum TH are affected in a time-dependent fashion after repeated social stressful stimuli and serotonergic system is implicated in these changes. Therefore, we aimed to investigate the possible alterations in thyroid hormone economy and type 1 (D1) and type 2 (D2) deiodinase activity in a model of social defeat stress. Thereafter, we tested the responsiveness of these changes to fluoxetine treatment. Both short (STS) and a long-term (LTS) stress were performed. Blood samples were drawn just before and 1 (STS) or 4 and 8 weeks (LTS) after the beginning of stress to assess serum T4, T3 and corticosterone. Deiodinases activity was assessed at the end of each protocol. Stress-induced behavior studied in open field arena and hypercorticosteronemia were mainly observed in LTS (week 4). Stress-induced behavior was associated to hypothyroidism which occurred before, since week 1 in stressed group. Serum TH was restored to control levels in week 8, when behavior changes were not observed anymore, and was mainly associated with high brown adipose tissue D2 activity since thyroid and liver D1 activity were low or normal in the STS and LTS respectively in stressed rats compared to control. Antidepressant study revealed that fluoxetine treatment (10mg/kg po during four weeks) fully reversed stress-induced behavior and normalized serum T4, but not T3 levels and hypercorticosteronemia in stressed group compared to control. The current work adds new concepts concerning TH metabolism changes induced by social stress and suggests that serotonergic system impairment may take part in the key events which ultimately lead to hypothyroxinemia and behavioral changes induced by chronic social defeat. This article is part of a Special Issue entitled ‘Anxiety and Depression’. ► The effects of stress on thyroid function depend on the kind and time of stress. ► We evaluated serum thyroid hormone in social defeated rats. ► Stress-like behavior followed hypothyroidism which occurred firstly during stress. ► This work suggests that hypothyroidism leads to behavioral changes observed in social defeated rats.