Biomarkers of cellular reaction to pesticide exposure in a rural population

In the present study we report data obtained from the evaluation of subjects occupationally exposed to pesticide mixtures from Santa Fe province, Argentina, using biomarkers for butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities, catalase (CAT), lipid peroxidation (by TBARS assa...

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Published inBiomarkers Vol. 15; no. 1; pp. 52 - 60
Main Authors Simoniello, María Fernanda, Kleinsorge, Elisa Carlotta, Scagnetti, Jorge Alberto, Mastandrea, Carlos, Grigolato, Raúl Alberto, Paonessa, Adriana Monica, Carballo, Marta Ana
Format Journal Article
LanguageEnglish
Published England Informa UK Ltd 01.02.2010
Taylor & Francis
Subjects
Acetylcholinesterase - analysis
Acetylcholinesterase - blood
Adult
Agricultural Workers' Diseases - blood
Agricultural Workers' Diseases - diagnosis
Agricultural Workers' Diseases - etiology
Argentina
Biomarkers - blood
Butyrylcholinesterase - analysis
Butyrylcholinesterase - blood
Case-Control Studies
Catalase - analysis
Catalase - blood
Comet Assay
DNA Damage
Female
genotoxicity
human biomonitoring
Humans
Lipid Peroxidation
Male
Middle Aged
Occupational Exposure - analysis
Oxidative stress
pesticides
Pesticides - adverse effects
Young Adult
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Summary:In the present study we report data obtained from the evaluation of subjects occupationally exposed to pesticide mixtures from Santa Fe province, Argentina, using biomarkers for butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities, catalase (CAT), lipid peroxidation (by TBARS assay) and the Damage Index Comet Assay (DICA). Our results showed an AChE inhibition (25% and 15% in directly and indirectly groups, respectively) in relation to controls with no significant modifications in BChE. TBARS levels were higher (51%) in pesticide sprayers while CAT activity was reduced in both, applicators (61%) and non-applicators (43%). DICA was significantly increased in direct (83%) and indirect (98%) exposed groups, compared with controls. These results showed modifications in lipid peroxidation, antioxidant defence system, and DNA damage in lymphocytes of exposed workers. Further investigations are suggested in order to link our findings with adverse health effects observed in chronic pesticide toxicity, where oxidative damage plays a pathophysiological role.
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ISSN:1354-750X
1366-5804
1366-5804
DOI:10.3109/13547500903276378