Halophilic Protein Stabilization by the Mild Solubilizing Agents Nondetergent Sulfobetaines

In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separa...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 230; no. 2; pp. 290 - 294
Main Authors Vuillard, L., Madern, D., Franzetti, B., Rabilloud, T.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 20.09.1995
Subjects
Animals
Betaine - pharmacology
Drug Stability
Enzyme Stability
Malate Dehydrogenase - chemistry
Peptide Elongation Factor Tu - chemistry
Swine
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Summary:In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1476