Supplementation with l-glutamine prevents tumor growth and cancer-induced cachexia as well as restores cell proliferation of intestinal mucosa of Walker-256 tumor-bearing rats

• Published in: Amino acids Vol. 48; no. 12; pp. 2773 - 2784
• Main Authors: Martins, Heber Amilcar, Sehaber, Camila Caviquioli, Hermes-Uliana, Catchia, Mariani, Fernando Augusto, Guarnier, Flavia Alessandra, Vicentini, Geraldo Emílio, Bossolani, Gleison Daion Piovezana, Jussani, Laraine Almeida, Lima, Mariana Machado, Bazotte, Roberto Barbosa, Zanoni, Jacqueline Nelisis
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.12.2016; Springer Nature B.V
• Subjects: Analytical Chemistry; Animals; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Cachexia - diet therapy; Cachexia - pathology; Carcinogenesis - drug effects; Cell Proliferation - drug effects; Dietary Supplements; Glutamine - pharmacology; Humans; Intestinal Mucosa - drug effects; Life Sciences; Neoplasms - diet therapy; Neoplasms - metabolism; Neoplasms - pathology; Neurobiology; Original Article; Proteomics; Rats; Rattus norvegicus; Cell proliferation; Cancer-associated cachexia; Tumor growth; Intestinal mucosa; Glutamine; L-Glutamine
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-016-2313-1

This study aimed to evaluate the intestinal mucosa of the duodenum and jejunum of Walker-256 tumor-bearing rats supplemented with l -glutamine. Thirty-two male 50-day-old Wistar rats ( Rattus norvegicus ) were randomly divided into four groups: control (C), control supplemented with 2 % l -glutamine (GC), Walker-256 tumor (WT), and Walker-256 tumor supplemented with 2 % l -glutamine (TWG). Walker-256 tumor was induced by inoculation viable tumor cells in the right rear flank. After 10 days, celiotomy was performed and duodenal and jejunal tissues were removed and processed. We evaluated the cachexia index, proliferation index, villus height, crypt depth, total height of the intestinal wall, and number of goblet cells by the technique of periodic acid-Schiff (PAS). Induction of Walker-256 tumor promoted a reduction of metaphase index in the TW group animals, which was accompanied by a reduction in the villous height and crypt depths, resulting in atrophy of the intestinal wall as well as increased PAS-positive goblet cells. Supplementation with l -glutamine reduced the tumor growth and inhibited the development of the cachectic syndrome in animals of the TWG group. Furthermore, amino acid supplementation promoted beneficial effects on the intestinal mucosa in the TWG animals through restoration of the number of PAS-positive goblet cells. Therefore, supplementation with 2 % l -glutamine exhibited a promising role in the prevention of tumor growth and cancer-associated cachexia as well as restoring the intestinal mucosa in the duodenum and jejunum of Walker-256 tumor-bearing rats.