Characterization of novel diesel-degrading strains Acinetobacter haemolyticus MJ01 and Acinetobacter johnsonii MJ4 isolated from oil-contaminated soil

• Published in: World journal of microbiology & biotechnology Vol. 28; no. 5; pp. 2057 - 2067
• Main Authors: Lee, Myungjin, Woo, Sung-Geun, Ten, Leonid N.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2012; Springer Nature B.V
• Subjects: Acinetobacter - classification; Acinetobacter - genetics; Acinetobacter - isolation & purification; Acinetobacter - metabolism; Acinetobacter haemolyticus; Acinetobacter johnsonii; Analysis; Applied Microbiology; Bacteria; Bacterial Typing Techniques; Biochemistry; Biodegradation; Biomedical and Life Sciences; Biotechnology; Biotransformation; Carbon; Cluster Analysis; Deoxyribonucleic acid; Diesel fuels; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Energy sources; Environmental Engineering/Biotechnology; Fatty acids; Fatty Acids - analysis; Gasoline; Hybridization; Hydrocarbons; Korea; Life Sciences; Microbiology; Microorganisms; Molecular Sequence Data; Nucleic Acid Hybridization; Oil pollution; Oils - metabolism; Original Paper; Phylogenetics; Phylogeny; Physiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil contamination; Soil Microbiology; Soil Pollutants; Studies; Time Factors; Korea; Korea, Rep; Biodegradation; MJ01; MJ4; Diesel oil
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-012-1008-3

The diesel-degrading strains, designated as MJ01 and MJ4, were isolated from oil-contaminated soil in Daejeon (South Korea) and were taxonomically characterized using a polyphasic approach and their diesel oil degradation abilities were analyzed. The isolates MJ01 and MJ4 were identified as Acinetobacter haemolyticus and Acinetobacter johnsonii , respectively, based on their 16S rDNA gene sequences, DNA–DNA relatedness, fatty acid profiles and various physiological characteristics. Strains MJ01 and MJ4 were able to use diesel oil as the sole carbon and energy source. Both strains could degrade over 90% of diesel oil with an initial concentration of 20,000 mg/l after incubation for 7 days, the most significant degradation occurred during the first 3 days. To our knowledge, this is the first report on diesel oil-degrading microorganisms among bacterial strains belonging to A. haemolyticus and A. johnsonii .